Detailed information of TA system TA119391

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 140680..141274
• Replicon: plasmid pS65EC
• Accession: NZ_CP036244
• Organism: Escherichia coli strain S65EC

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: S65EC_RS00940
• Protein ID: WP_001403920.1
• Coordinates: 140680..140955 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: G3CAJ1
• Locus tag: S65EC_RS00945
• Protein ID: WP_001261274.1
• Coordinates: 141044..141274 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
S65EC_RS00900	135760..136067	+	308	Protein_164	type II toxin-antitoxin system ParD family antitoxin	-
S65EC_RS00905	136183..136344	+	162	WP_001440647.1	type II toxin-antitoxin system RelE/ParE family toxin	-
S65EC_RS00910	136411..136593	+	183	WP_042065278.1	hypothetical protein	-
S65EC_RS00915	136714..137454	+	741	WP_001066940.1	tyrosine-type recombinase/integrase	-
S65EC_RS00920	137777..138766	-	990	WP_024201093.1	RepB family plasmid replication initiator protein	-
S65EC_RS00925	139654..139923	+	270	WP_001275013.1	DUF1778 domain-containing protein	-
S65EC_RS00930	139927..140457	+	531	WP_000348883.1	GNAT family N-acetyltransferase	-
S65EC_RS00935	140458..140675	+	218	Protein_171	transposase	-
S65EC_RS00940	140680..140955	-	276	WP_001403920.1	type II toxin-antitoxin system VapC family toxin	Toxin
S65EC_RS00945	141044..141274	-	231	WP_001261274.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
S65EC_RS00950	141473..144094	-	2622	WP_000283688.1	histidinol-phosphatase	-
S65EC_RS00955	144485..144976	+	492	WP_000528930.1	hypothetical protein	-
S65EC_RS00960	144988..145746	+	759	WP_032174241.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..146792	146792

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• SpoVT-AbrB domain (7-45)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 9621.03 Da
• Isoelectric Point: 8.5444

>T119391 WP_001403920.1 NZ_CP036244:c140955-140680 [Escherichia coli]
VFSHRIVVSAITYSEMRFGASGPKASPRHVQLVDKFCPCLDAILSWDRAAVDGTTDIKVALRQAGTPIGQNDTAIAGPEA
GRLGKLTSPTL

Nucleotide

• Length: 276 bp

>T119391 NZ_CP036244:c140955-140680 [Escherichia coli]
GTGTTTAGTCACCGCATTGTGGTCTCGGCCATTACTTACTCTGAAATGCGCTTCGGTGCCTCCGGTCCGAAGGCCTCCCC
GCGTCACGTGCAACTGGTTGATAAATTCTGCCCGTGCCTCGATGCCATCCTGTCTTGGGACCGCGCCGCGGTGGACGGCA
CCACGGATATTAAAGTGGCACTGCGCCAGGCCGGGACGCCGATCGGCCAGAACGATACGGCGATTGCCGGGCCTGAAGCT
GGAAGACTGGGTAAATTAACATCGCCCACACTCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8856.95 Da
• Isoelectric Point: 4.6286

>AT119391 WP_001261274.1 NZ_CP036244:c141274-141044 [Escherichia coli]
MRTVSIFKNGNNRAIHLPRDLDFERVRELEIVREGDSIILRPVQPNWGSFAQLEKADPDFMAERENVVSDEGRFDL

Nucleotide

• Length: 231 bp

>AT119391 NZ_CP036244:c141274-141044 [Escherichia coli]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATCCATCTGCCCCGCGATTTGGATTTTGAGCGGGTGAG
AGAGCTGGAGATCGTCCGCGAAGGAGACAGCATCATCCTGCGTCCCGTCCAGCCGAACTGGGGCTCATTCGCGCAGCTTG
AAAAAGCCGATCCGGATTTTATGGCGGAGCGCGAGAATGTTGTCAGCGACGAAGGTCGATTTGACCTGTGA

Structures

Antitoxin

• AlphaFold DB: G3CAJ1