Detailed information of TA system TA119390

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/ElaA-DUF1778
• Location: 139654..140457
• Replicon: plasmid pS65EC
• Accession: NZ_CP036244
• Organism: Escherichia coli strain S65EC

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: G3CAI8
• Locus tag: S65EC_RS00930
• Protein ID: WP_000348883.1
• Coordinates: 139927..140457 (+)
• Length: 177 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: G3CAI7
• Locus tag: S65EC_RS00925
• Protein ID: WP_001275013.1
• Coordinates: 139654..139923 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
S65EC_RS00900	135760..136067	+	308	Protein_164	type II toxin-antitoxin system ParD family antitoxin	-
S65EC_RS00905	136183..136344	+	162	WP_001440647.1	type II toxin-antitoxin system RelE/ParE family toxin	-
S65EC_RS00910	136411..136593	+	183	WP_042065278.1	hypothetical protein	-
S65EC_RS00915	136714..137454	+	741	WP_001066940.1	tyrosine-type recombinase/integrase	-
S65EC_RS00920	137777..138766	-	990	WP_024201093.1	RepB family plasmid replication initiator protein	-
S65EC_RS00925	139654..139923	+	270	WP_001275013.1	DUF1778 domain-containing protein	Antitoxin
S65EC_RS00930	139927..140457	+	531	WP_000348883.1	GNAT family N-acetyltransferase	Toxin
S65EC_RS00935	140458..140675	+	218	Protein_171	transposase	-
S65EC_RS00940	140680..140955	-	276	WP_001403920.1	type II toxin-antitoxin system VapC family toxin	-
S65EC_RS00945	141044..141274	-	231	WP_001261274.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
S65EC_RS00950	141473..144094	-	2622	WP_000283688.1	histidinol-phosphatase	-
S65EC_RS00955	144485..144976	+	492	WP_000528930.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..146792	146792

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (92-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 177 a.a.
• Molecular weight: 20321.20 Da
• Isoelectric Point: 6.4889

>T119390 WP_000348883.1 NZ_CP036244:139927-140457 [Escherichia coli]
MDGLRIEIFSEEVEYQLSNFDCGEEYLNTFLTDHLQRQHSSKILRGYLLVTREDRPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCDSL
FYPTKSIEVLFEVNDE

Nucleotide

• Length: 531 bp

>T119390 NZ_CP036244:139927-140457 [Escherichia coli]
ATGGACGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATCAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTCACCGATCATCTGCAACGTCAGCACAGCAGTAAGATTTTACGGGGTTATCTGCTCGTGACGAGAGAGG
ATAGACCACGTGTCATGGGGTACTACACCCTGTCAGGCAGTTGCTTTGAGAAAATACTTCTCCCCAGCAAAACACAACAA
AAGCGTGTGCCCTACAAAAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCATCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AGGCGCTGAACGATAACGCCAAAAAATTCTACCTTCGACTGGGTTTTATACAGTTAAAGGAAGAAAACTGTGATTCGCTC
TTCTACCCGACAAAATCTATTGAAGTATTGTTCGAAGTAAATGACGAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10229.63 Da
• Isoelectric Point: 6.4702

>AT119390 WP_001275013.1 NZ_CP036244:139654-139923 [Escherichia coli]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASARAAEVIDQHRRLLLNEESWNLVMDAITNPPAPNDRLKR
ATNRLRELE

Nucleotide

• Length: 270 bp

>AT119390 NZ_CP036244:139654-139923 [Escherichia coli]
ATGTCAGCACTGAAGAAGCAGCGCATCGATCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCCATTTCGCAGTTCATGGTCAGTACCGCCTCAGCACGTGCTGCTGAAGTTATTGATCAGCACCGTCGCC
TGCTCCTCAATGAGGAGTCCTGGAATCTGGTTATGGATGCAATCACTAATCCGCCAGCACCAAATGACCGGCTGAAACGC
GCCACTAACCGTCTCCGGGAACTGGAGTAA

Structures

Toxin

• AlphaFold DB: G3CAI8

Antitoxin

• AlphaFold DB: G3CAI7