Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 132944..133503 Replicon plasmid pS65EC
Accession NZ_CP036244
Organism Escherichia coli strain S65EC

Toxin (Protein)


Gene name relE Uniprot ID V0SVC8
Locus tag S65EC_RS00875 Protein ID WP_000421272.1
Coordinates 133228..133503 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SRR3
Locus tag S65EC_RS00870 Protein ID WP_001387467.1
Coordinates 132944..133228 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
S65EC_RS00840 128407..128847 - 441 Protein_154 zinc-binding dehydrogenase -
S65EC_RS00850 129248..130138 + 891 WP_001077068.1 dihydrodipicolinate synthase family protein -
S65EC_RS00855 130163..130543 + 381 WP_000710783.1 Rid family detoxifying hydrolase -
S65EC_RS00860 130576..131541 + 966 WP_001022265.1 2-keto-3-deoxygluconate transporter -
S65EC_RS00865 131587..132339 - 753 WP_001403368.1 IclR family transcriptional regulator -
S65EC_RS00870 132944..133228 + 285 WP_001387467.1 ribbon-helix-helix domain-containing protein Antitoxin
S65EC_RS00875 133228..133503 + 276 WP_000421272.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
S65EC_RS00880 133609..133902 + 294 WP_086732350.1 hypothetical protein -
S65EC_RS00885 133937..134065 - 129 Protein_162 IS481 family transposase -
S65EC_RS00890 134098..135267 - 1170 WP_086732351.1 hypothetical protein -
S65EC_RS00900 135760..136067 + 308 Protein_164 type II toxin-antitoxin system ParD family antitoxin -
S65EC_RS00905 136183..136344 + 162 WP_001440647.1 type II toxin-antitoxin system RelE/ParE family toxin -
S65EC_RS00910 136411..136593 + 183 WP_042065278.1 hypothetical protein -
S65EC_RS00915 136714..137454 + 741 WP_001066940.1 tyrosine-type recombinase/integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..146792 146792


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 10.0871

>T119389 WP_000421272.1 NZ_CP036244:133228-133503 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T119389 NZ_CP036244:133228-133503 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10460.87 Da        Isoelectric Point: 5.6558

>AT119389 WP_001387467.1 NZ_CP036244:132944-133228 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Download         Length: 285 bp

>AT119389 NZ_CP036244:132944-133228 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SVC8

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y880

References