Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 15386..15987 Replicon plasmid pS65EC
Accession NZ_CP036244
Organism Escherichia coli strain S65EC

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag S65EC_RS00105 Protein ID WP_001216034.1
Coordinates 15386..15766 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag S65EC_RS00110 Protein ID WP_001190712.1
Coordinates 15766..15987 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
S65EC_RS00070 10437..11369 - 933 WP_000081352.1 homocysteine S-methyltransferase -
S65EC_RS00075 11356..12759 - 1404 WP_001373486.1 S-methylmethionine permease -
S65EC_RS00080 13003..13983 + 981 WP_000019407.1 IS5-like element IS5 family transposase -
S65EC_RS00085 14211..14528 + 318 WP_001513659.1 hypothetical protein -
S65EC_RS00090 14478..14891 - 414 Protein_15 DDE-type integrase/transposase/recombinase -
S65EC_RS00095 14815..15174 - 360 WP_001513660.1 hypothetical protein -
S65EC_RS00100 15202..15381 - 180 WP_001513661.1 hypothetical protein -
S65EC_RS00105 15386..15766 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
S65EC_RS00110 15766..15987 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
S65EC_RS00115 16170..17726 + 1557 WP_001617892.1 type I restriction-modification system subunit M -
S65EC_RS00120 17723..19006 + 1284 WP_001617890.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..146792 146792


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T119380 WP_001216034.1 NZ_CP036244:c15766-15386 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T119380 NZ_CP036244:c15766-15386 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT119380 WP_001190712.1 NZ_CP036244:c15987-15766 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT119380 NZ_CP036244:c15987-15766 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References