Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 54355..54890 Replicon plasmid punnamed2
Accession NZ_CP036204
Organism Escherichia coli strain L725

Toxin (Protein)


Gene name relE Uniprot ID A0A0K4CX11
Locus tag D9K49_RS24630 Protein ID WP_000222771.1
Coordinates 54355..54642 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID T1PXV2
Locus tag D9K49_RS24635 Protein ID WP_001139206.1
Coordinates 54639..54890 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D9K49_RS25440 49875..49997 + 123 Protein_59 IS66 family insertion sequence element accessory protein TnpB -
D9K49_RS24595 50089..50870 - 782 Protein_60 IS21-like element helper ATPase IstB -
D9K49_RS24600 50867..51475 - 609 Protein_61 transposase -
D9K49_RS24605 51489..51861 - 373 Protein_62 IS1 family transposase -
D9K49_RS24610 52188..52885 - 698 Protein_63 IS1 family transposase -
D9K49_RS24615 52936..53633 - 698 Protein_64 IS1 family transposase -
D9K49_RS24620 53667..53875 - 209 Protein_65 hypothetical protein -
D9K49_RS24625 53865..54065 + 201 WP_000896262.1 DUF839 domain-containing protein -
D9K49_RS24630 54355..54642 - 288 WP_000222771.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
D9K49_RS24635 54639..54890 - 252 WP_001139206.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
D9K49_RS24645 55503..59387 + 3885 WP_130723510.1 helicase -
D9K49_RS24650 59421..59861 + 441 WP_023352064.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid oqxA / blaCTX-M-55 - 1..65959 65959
- inside IScluster/Tn - - 39635..53633 13998


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11116.17 Da        Isoelectric Point: 10.5833

>T119372 WP_000222771.1 NZ_CP036204:c54642-54355 [Escherichia coli]
MTYTVKFRDDALKEWLRLDKTIQQQFAKKLKKCSENPHIASAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYHLASERMK

Download         Length: 288 bp

>T119372 NZ_CP036204:c54642-54355 [Escherichia coli]
ATGACTTATACAGTAAAATTCCGGGATGATGCACTGAAAGAATGGCTCAGACTGGACAAGACCATTCAGCAGCAGTTCGC
AAAAAAACTGAAAAAATGCAGTGAAAATCCTCATATAGCTTCTGCAAAACTACGTGGATTAAAGGACTGCTATAAAATCA
AACTTCGCGCATCAGGTTTTCGTCTGGTTTATCAGGTTATTGATGATATGTTAATTATTGCGGTCGTCGCAGTAGGTAAA
CGTGAACGCAGCAACGTTTATCATCTCGCCAGTGAACGGATGAAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9171.39 Da        Isoelectric Point: 4.0927

>AT119372 WP_001139206.1 NZ_CP036204:c54890-54639 [Escherichia coli]
MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSSQPLHDVDLD
SYL

Download         Length: 252 bp

>AT119372 NZ_CP036204:c54890-54639 [Escherichia coli]
ATGCCCAGTATCATTTTGTCTGACACCAGTGCAAGCGTCAGTGAGCTGAAAAAAAATCCGATGGCAACTGTCAGTGCCGG
AGAGGGGTATCCTGTTGCCATTCTTAACCGGAATCAGCCAGCATTCTACTGTGTCCCTGCTGAGCTCTATGAAAGAATGC
TTGATGCCTTGGATGACCAGGAGCTGGTAAAACTGGTTACTGAACGCAGCAGTCAGCCTCTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K4CX11

Antitoxin

Source ID Structure
AlphaFold DB T1PXV2

References