119371

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 48033..48559 Replicon plasmid punnamed2
Accession NZ_CP036204
Organism Escherichia coli strain L725

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag D9K49_RS24575 Protein ID WP_000323025.1
Coordinates 48033..48320 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag D9K49_RS24580 Protein ID WP_000534858.1
Coordinates 48320..48559 (-) Length 80 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D9K49_RS24540 43431..43586 + 156 WP_000587091.1 hypothetical protein -
D9K49_RS24545 43637..44749 + 1113 WP_001300563.1 IS4-like element IS421 family transposase -
D9K49_RS24550 44922..46304 + 1383 Protein_51 group II intron reverse transcriptase/maturase -
D9K49_RS24555 46385..46852 + 468 Protein_52 IS3 family transposase -
D9K49_RS24560 46883..47089 - 207 Protein_53 hypothetical protein -
D9K49_RS24565 47526..47750 - 225 Protein_54 hypothetical protein -
D9K49_RS24575 48033..48320 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
D9K49_RS24580 48320..48559 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
D9K49_RS25310 48901..49050 + 150 WP_000550473.1 hypothetical protein -
D9K49_RS24585 49107..49811 - 705 WP_001067858.1 IS6-like element IS26 family transposase -
D9K49_RS25440 49875..49997 + 123 Protein_59 IS66 family insertion sequence element accessory protein TnpB -
D9K49_RS24595 50089..50870 - 782 Protein_60 IS21-like element helper ATPase IstB -
D9K49_RS24600 50867..51475 - 609 Protein_61 transposase -
D9K49_RS24605 51489..51861 - 373 Protein_62 IS1 family transposase -
D9K49_RS24610 52188..52885 - 698 Protein_63 IS1 family transposase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid oqxA / blaCTX-M-55 - 1..65959 65959
- inside IScluster/Tn - - 39635..53633 13998


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T119371 WP_000323025.1 NZ_CP036204:c48320-48033 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T119371 NZ_CP036204:c48320-48033 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT119371 WP_000534858.1 NZ_CP036204:c48559-48320 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT119371 NZ_CP036204:c48559-48320 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9


Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References