Detailed information of TA system TA119371

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 48033..48559
• Replicon: plasmid punnamed2
• Accession: NZ_CP036204
• Organism: Escherichia coli strain L725

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: D9K49_RS24575
• Protein ID: WP_000323025.1
• Coordinates: 48033..48320 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: D9K49_RS24580
• Protein ID: WP_000534858.1
• Coordinates: 48320..48559 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9K49_RS24540	43431..43586	+	156	WP_000587091.1	hypothetical protein	-
D9K49_RS24545	43637..44749	+	1113	WP_001300563.1	IS4-like element IS421 family transposase	-
D9K49_RS24550	44922..46304	+	1383	Protein_51	group II intron reverse transcriptase/maturase	-
D9K49_RS24555	46385..46852	+	468	Protein_52	IS3 family transposase	-
D9K49_RS24560	46883..47089	-	207	Protein_53	hypothetical protein	-
D9K49_RS24565	47526..47750	-	225	Protein_54	hypothetical protein	-
D9K49_RS24575	48033..48320	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
D9K49_RS24580	48320..48559	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
D9K49_RS25310	48901..49050	+	150	WP_000550473.1	hypothetical protein	-
D9K49_RS24585	49107..49811	-	705	WP_001067858.1	IS6-like element IS26 family transposase	-
D9K49_RS25440	49875..49997	+	123	Protein_59	IS66 family insertion sequence element accessory protein TnpB	-
D9K49_RS24595	50089..50870	-	782	Protein_60	IS21-like element helper ATPase IstB	-
D9K49_RS24600	50867..51475	-	609	Protein_61	transposase	-
D9K49_RS24605	51489..51861	-	373	Protein_62	IS1 family transposase	-
D9K49_RS24610	52188..52885	-	698	Protein_63	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	oqxA / blaCTX-M-55	-	1..65959	65959
	-	inside	IScluster/Tn	-	-	39635..53633	13998

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T119371 WP_000323025.1 NZ_CP036204:c48320-48033 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T119371 NZ_CP036204:c48320-48033 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT119371 WP_000534858.1 NZ_CP036204:c48559-48320 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT119371 NZ_CP036204:c48559-48320 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8