Detailed information of TA system TA119370

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 13641..14242
• Replicon: plasmid punnamed2
• Accession: NZ_CP036204
• Organism: Escherichia coli strain L725

Toxin (Protein)

• Gene name: doc
• Uniprot ID: F0JYA5
• Locus tag: D9K49_RS24330
• Protein ID: WP_001216044.1
• Coordinates: 13641..14021 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: D9K49_RS24335
• Protein ID: WP_001190712.1
• Coordinates: 14021..14242 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9K49_RS24290	9491..10207	+	717	Protein_7	Tn3 family transposase	-
D9K49_RS24295	10326..11023	-	698	Protein_8	IS1 family transposase	-
D9K49_RS24310	11214..11423	+	210	WP_000874154.1	hypothetical protein	-
D9K49_RS24315	11534..12385	+	852	WP_000611653.1	hypothetical protein	-
D9K49_RS24320	12560..13429	-	870	Protein_11	DUF968 domain-containing protein	-
D9K49_RS24325	13457..13636	-	180	WP_039022005.1	hypothetical protein	-
D9K49_RS24330	13641..14021	-	381	WP_001216044.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
D9K49_RS24335	14021..14242	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
D9K49_RS24340	14315..14704	-	390	WP_000506730.1	DNA repair protein	-
D9K49_RS24345	14879..15454	+	576	WP_039022006.1	hypothetical protein	-
D9K49_RS24350	15461..15712	-	252	WP_039022007.1	DNA polymerase III subunit theta	-
D9K49_RS24355	16502..16717	-	216	WP_024259737.1	hypothetical protein	-
D9K49_RS24360	16838..17587	-	750	WP_205694909.1	hypothetical protein	-
D9K49_RS24365	17569..17943	-	375	WP_039022008.1	hypothetical protein	-
D9K49_RS25300	18010..18174	-	165	WP_152950163.1	hypothetical protein	-
D9K49_RS24370	18174..18467	-	294	WP_039022009.1	hypothetical protein	-
D9K49_RS24375	18646..18879	-	234	WP_065311123.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	oqxA / blaCTX-M-55	-	1..65959	65959

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13544.28 Da
• Isoelectric Point: 5.6343

>T119370 WP_001216044.1 NZ_CP036204:c14021-13641 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVAATLRRLYGSAE

Nucleotide

• Length: 381 bp

>T119370 NZ_CP036204:c14021-13641 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGCCACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT119370 WP_001190712.1 NZ_CP036204:c14242-14021 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT119370 NZ_CP036204:c14242-14021 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: A0A6Y6HMN5

Antitoxin

• AlphaFold DB: A0A829CJB6