Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 3996845..3997483 Replicon chromosome
Accession NZ_CP036202
Organism Escherichia coli strain L725

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag D9K49_RS19450 Protein ID WP_000813794.1
Coordinates 3997307..3997483 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag D9K49_RS19445 Protein ID WP_001270286.1
Coordinates 3996845..3997261 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D9K49_RS19425 3991997..3992938 - 942 WP_001251313.1 ABC transporter permease -
D9K49_RS19430 3992939..3993952 - 1014 WP_001542893.1 ABC transporter ATP-binding protein -
D9K49_RS19435 3993970..3995115 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
D9K49_RS19440 3995360..3996766 - 1407 WP_000760663.1 PLP-dependent aminotransferase family protein -
D9K49_RS19445 3996845..3997261 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
D9K49_RS19450 3997307..3997483 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
D9K49_RS19455 3997705..3997935 + 231 WP_000494244.1 YncJ family protein -
D9K49_RS19460 3998027..3999988 - 1962 WP_130723458.1 23S rRNA 5-hydroxycytidine C2501 synthase -
D9K49_RS19465 4000061..4000597 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
D9K49_RS19470 4000650..4001864 + 1215 WP_071609973.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4001904..4003052 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T119365 WP_000813794.1 NZ_CP036202:c3997483-3997307 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T119365 NZ_CP036202:c3997483-3997307 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT119365 WP_001270286.1 NZ_CP036202:c3997261-3996845 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT119365 NZ_CP036202:c3997261-3996845 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References