Detailed information of TA system TA119365

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 3996845..3997483
• Replicon: chromosome
• Accession: NZ_CP036202
• Organism: Escherichia coli strain L725

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: D9K49_RS19450
• Protein ID: WP_000813794.1
• Coordinates: 3997307..3997483 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: D9K49_RS19445
• Protein ID: WP_001270286.1
• Coordinates: 3996845..3997261 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9K49_RS19425	3991997..3992938	-	942	WP_001251313.1	ABC transporter permease	-
D9K49_RS19430	3992939..3993952	-	1014	WP_001542893.1	ABC transporter ATP-binding protein	-
D9K49_RS19435	3993970..3995115	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
D9K49_RS19440	3995360..3996766	-	1407	WP_000760663.1	PLP-dependent aminotransferase family protein	-
D9K49_RS19445	3996845..3997261	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
D9K49_RS19450	3997307..3997483	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
D9K49_RS19455	3997705..3997935	+	231	WP_000494244.1	YncJ family protein	-
D9K49_RS19460	3998027..3999988	-	1962	WP_130723458.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
D9K49_RS19465	4000061..4000597	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
D9K49_RS19470	4000650..4001864	+	1215	WP_071609973.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4001904..4003052	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T119365 WP_000813794.1 NZ_CP036202:c3997483-3997307 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T119365 NZ_CP036202:c3997483-3997307 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT119365 WP_001270286.1 NZ_CP036202:c3997261-3996845 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT119365 NZ_CP036202:c3997261-3996845 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB