119346

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) sanaTA/AbiEii-DUF6088
Location 851128..852463 Replicon chromosome
Accession NZ_CP036202
Organism Escherichia coli strain L725

Toxin (Protein)


Gene name sanaT Uniprot ID F4VMR9
Locus tag D9K49_RS04165 Protein ID WP_000342497.1
Coordinates 851128..852075 (-) Length 316 a.a.

Antitoxin (Protein)


Gene name sanaA Uniprot ID A0A228Z6F8
Locus tag D9K49_RS04170 Protein ID WP_000152739.1
Coordinates 852068..852463 (-) Length 132 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D9K49_RS04135 846981..847697 + 717 WP_001295734.1 N-acetylneuraminic acid outer membrane channel NanC -
D9K49_RS04140 847717..848823 + 1107 WP_001044128.1 N-acetylneuraminate epimerase -
D9K49_RS04145 848888..849868 + 981 WP_001366032.1 9-O-acetyl-N-acetylneuraminic acid deacetylase -
D9K49_RS04150 849876..850526 - 651 WP_001037970.1 HNH endonuclease -
D9K49_RS04160 850905..851090 + 186 WP_000066585.1 hypothetical protein -
D9K49_RS04165 851128..852075 - 948 WP_000342497.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein Toxin
D9K49_RS04170 852068..852463 - 396 WP_000152739.1 type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein Antitoxin
D9K49_RS04175 852533..853366 - 834 WP_001192741.1 DUF4942 domain-containing protein -
D9K49_RS04180 853672..855228 + 1557 WP_000202816.1 type I restriction-modification system subunit M -
D9K49_RS04185 855225..856391 + 1167 WP_001293040.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - fimI / fimA / fimE / fimB 842215..856391 14176


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Nucleotidyl transferase AbiEii toxin, Type IV TA system

(38-303)

Antitoxin

Protein of unknown function DUF6088

(7-108)


Sequences

Toxin        


Download         Length: 316 a.a.        Molecular weight: 35588.79 Da        Isoelectric Point: 4.9737

>T119346 WP_000342497.1 NZ_CP036202:c852075-851128 [Escherichia coli]
MDDLTTLFSEVADALGIESVAIVEKDHYIVELLQLLKPLVFDTHQLVFAGGTALAKSGVALNRMSEDVDIKLVPKDAFQA
LSRTQRKNTRKEIVQAVSSAIASSGVFNFDDDYPKVTRDEYRYNDIPVRYPQRVVQAPCLRPFIRLELMEAKLLEPSELR
DIHSLVTGITGKAAIVSAFPCATIISTQAEKLVAMLRRTAAVVRDVERFDDASLVRHIYDTNCIVETSHVDMSLLVDFVQ
QTIEQDIQRYGNQYPQFCASPVDELKMGLDELANNPLYQMRYQQFVSPMVYGERKITWDEAYSRFRSLALAILNA

Download         Length: 948 bp

>T119346 NZ_CP036202:c852075-851128 [Escherichia coli]
ATGGATGATTTAACCACTCTTTTTTCTGAAGTGGCTGACGCGCTTGGTATTGAGTCTGTCGCAATTGTGGAAAAAGATCA
CTATATCGTTGAGCTTTTGCAATTGCTTAAACCTCTGGTGTTCGATACTCATCAATTGGTGTTTGCCGGAGGAACCGCTC
TGGCAAAATCAGGTGTCGCACTTAATCGCATGTCGGAAGATGTGGATATCAAACTGGTGCCTAAGGATGCATTTCAGGCA
CTTTCCCGCACTCAGCGAAAAAATACCCGTAAAGAAATTGTTCAGGCAGTTTCCAGTGCAATAGCTTCATCGGGTGTGTT
CAATTTTGATGATGATTACCCAAAAGTGACGCGGGATGAATATCGTTATAATGACATACCTGTTCGTTATCCACAGCGAG
TAGTGCAGGCACCTTGTTTGCGTCCTTTTATCCGGCTGGAACTGATGGAAGCGAAGTTACTGGAGCCTTCTGAATTACGG
GATATTCATTCACTGGTAACAGGGATCACGGGAAAAGCGGCTATTGTTTCAGCGTTTCCATGCGCAACTATCATTAGCAC
ACAGGCAGAAAAGCTGGTGGCGATGTTACGTCGTACTGCGGCAGTTGTGCGAGATGTTGAGCGATTTGATGATGCATCGC
TGGTACGTCATATCTACGATACAAACTGTATTGTTGAGACGAGTCATGTTGATATGAGTCTGCTCGTTGATTTTGTTCAA
CAAACTATTGAGCAGGACATTCAGCGTTACGGCAACCAGTACCCGCAGTTTTGCGCGTCTCCAGTTGATGAGCTAAAAAT
GGGACTGGATGAGTTGGCGAACAATCCTCTCTACCAGATGCGCTATCAGCAGTTTGTTTCGCCAATGGTGTATGGAGAGC
GAAAGATCACATGGGATGAGGCTTACTCCAGGTTTAGGAGCTTAGCGCTAGCCATCCTGAATGCTTGA

Antitoxin


Download         Length: 132 a.a.        Molecular weight: 14605.90 Da        Isoelectric Point: 11.6915

>AT119346 WP_000152739.1 NZ_CP036202:c852463-852068 [Escherichia coli]
MTIKARIQSRLKRSKRYVFTRDDFKDIAGYDQVGRVLRDLVREGQLLKVGYGVYTKARRNGITGKVMPAAPGGSSAVIVE
TLERLKVRYRFAGATQAYNSGTSTQIPASPEIKTPRRFKRVLSVGNSRLNG

Download         Length: 396 bp

>AT119346 NZ_CP036202:c852463-852068 [Escherichia coli]
ATGACGATAAAAGCACGCATTCAGTCACGACTGAAACGTTCTAAACGTTACGTATTCACTCGTGACGACTTTAAAGATAT
TGCAGGCTACGATCAGGTTGGTCGGGTATTGCGCGATCTTGTCAGGGAAGGGCAACTGTTGAAGGTTGGTTATGGCGTGT
ATACCAAAGCACGGCGGAACGGTATTACAGGCAAGGTAATGCCAGCGGCTCCCGGAGGCTCTTCAGCGGTGATTGTCGAA
ACGCTGGAACGCCTGAAAGTACGCTATCGTTTTGCTGGCGCAACGCAGGCATACAATAGCGGTACTTCTACGCAGATCCC
AGCTTCACCCGAGATAAAAACCCCCCGGCGTTTTAAACGCGTATTATCTGTAGGGAACAGCAGATTAAATGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1L5BAQ6

Antitoxin

Source ID Structure
AlphaFold DB A0A228Z6F8

References