Detailed information of TA system TA119328

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 182118..182788
• Replicon: plasmid pvirulence_VBA34918
• Accession: NZ_CP036191
• Organism: Klebsiella pneumoniae strain BA34918

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: EX353_RS28060
• Protein ID: WP_130628009.1
• Coordinates: 182345..182788 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: EX353_RS28055
• Protein ID: WP_004213073.1
• Coordinates: 182118..182348 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EX353_RS28030	178349..179248	-	900	WP_004225022.1	nucleotide-binding protein	-
EX353_RS28035	179238..179528	-	291	WP_004213078.1	hypothetical protein	-
EX353_RS28040	179879..180085	+	207	WP_004213077.1	hypothetical protein	-
EX353_RS28045	180075..180368	-	294	WP_004213076.1	hypothetical protein	-
EX353_RS28050	180384..181517	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
EX353_RS28055	182118..182348	+	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
EX353_RS28060	182345..182788	+	444	WP_130628009.1	PIN domain-containing protein	Toxin
EX353_RS28065	182802..183218	-	417	Protein_194	restriction endonuclease	-
EX353_RS28070	183222..183515	-	294	Protein_195	transposase	-
EX353_RS32505	183932..184562	+	631	Protein_196	response regulator	-
EX353_RS28090	185077..185483	-	407	Protein_197	GAF domain-containing protein	-
EX353_RS28100	185574..186494	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
EX353_RS28105	186543..187034	-	492	WP_011251327.1	DM13 domain-containing protein	-
EX353_RS28110	187097..187372	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	catA1	iroN / iroD / iroC / iroB / rmpA2 / iutA / iucD / iucC / iucB / iucA / rmpA	1..216586	216586

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 15976.72 Da
• Isoelectric Point: 9.9117

>T119328 WP_130628009.1 NZ_CP036191:182345-182788 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPRDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T119328 NZ_CP036191:182345-182788 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCCGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT119328 WP_004213073.1 NZ_CP036191:182118-182348 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT119328 NZ_CP036191:182118-182348 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Antitoxin

• AlphaFold DB: Q6U620