Detailed information of TA system TA119310

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 129000..129745
• Replicon: plasmid pIncFIBK
• Accession: NZ_CP036188
• Organism: Klebsiella pneumoniae strain BA1559

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A331KSM6
• Locus tag: EX352_RS27540
• Protein ID: WP_032408901.1
• Coordinates: 129000..129491 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: EX352_RS27545
• Protein ID: WP_014386183.1
• Coordinates: 129479..129745 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EX352_RS27515	124685..125404	+	720	WP_130576733.1	DeoR/GlpR family DNA-binding transcription regulator	-
EX352_RS27520	125701..126141	+	441	WP_014386179.1	IS66 family insertion sequence hypothetical protein	-
EX352_RS27525	126138..126488	+	351	WP_014386180.1	IS66 family insertion sequence element accessory protein TnpB	-
EX352_RS27530	126519..128111	+	1593	Protein_134	IS66 family transposase	-
EX352_RS27535	128308..128559	+	252	WP_032408900.1	aldo/keto reductase	-
EX352_RS27540	129000..129491	-	492	WP_032408901.1	GNAT family N-acetyltransferase	Toxin
EX352_RS27545	129479..129745	-	267	WP_014386183.1	DUF1778 domain-containing protein	Antitoxin
EX352_RS27550	129942..130100	+	159	WP_014386184.1	type I toxin-antitoxin system Hok family toxin	-
EX352_RS27555	130343..130828	-	486	WP_014386185.1	hypothetical protein	-
EX352_RS27560	131018..131290	+	273	WP_032408902.1	hypothetical protein	-
EX352_RS27565	131287..131637	+	351	WP_014386186.1	hypothetical protein	-
EX352_RS27580	132274..132600	+	327	WP_014386187.1	hypothetical protein	-
EX352_RS27585	132660..132872	+	213	WP_014386188.1	hypothetical protein	-
EX352_RS27590	132883..133107	+	225	WP_014386189.1	hypothetical protein	-
EX352_RS27595	133188..133508	+	321	WP_014386190.1	type II toxin-antitoxin system RelE/ParE family toxin	-
EX352_RS27600	133498..133776	+	279	WP_004152721.1	helix-turn-helix domain-containing protein	-
EX352_RS27605	133777..134190	+	414	WP_013023817.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(A) / aph(3')-Ia / mph(A) / sul1 / qacE / aadA2 / dfrA12 / blaTEM-1B / blaCTX-M-15 / aac(6')-Ib-cr / blaOXA-1	-	1..251327	251327

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (89-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17667.46 Da
• Isoelectric Point: 8.8757

>T119310 WP_032408901.1 NZ_CP036188:c129491-129000 [Klebsiella pneumoniae]
VGKVTAPVPLGTSHILAEFHCGEPVLDEWIKHRGLKNQSLGAARTFVVCRGNASQVLAYYSLATGSVTHAIAPGSLRRNM
PDPVPVIILARLAVDTSYHGQGLGADLLHDAVLRCCRVAENIGVRAVMVHALSDSARQFYIHHGFIPSQTQDRTLFLRLP
LIT

Nucleotide

• Length: 492 bp

>T119310 NZ_CP036188:c129491-129000 [Klebsiella pneumoniae]
GTGGGCAAAGTAACGGCTCCGGTCCCACTCGGTACGTCACACATCCTCGCTGAGTTTCACTGTGGTGAGCCTGTACTGGA
CGAATGGATAAAGCACCGGGGACTAAAAAATCAGTCCCTGGGGGCGGCCAGAACGTTTGTGGTGTGCCGGGGAAATGCTT
CACAGGTGCTTGCCTACTACTCTCTCGCGACCGGCAGCGTCACTCATGCCATTGCGCCGGGAAGCCTGCGAAGGAATATG
CCCGATCCTGTACCTGTCATCATCCTGGCCCGTCTTGCTGTCGATACGAGTTATCACGGCCAGGGACTGGGGGCTGATTT
ACTGCATGATGCCGTATTACGTTGCTGTCGGGTGGCTGAGAACATTGGCGTGCGGGCCGTTATGGTTCATGCACTCTCTG
ACAGTGCCAGACAGTTCTACATACATCACGGATTTATCCCTTCACAAACGCAGGATAGAACCCTTTTTTTACGGTTGCCT
CTCATTACTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10090.66 Da
• Isoelectric Point: 5.1169

>AT119310 WP_014386183.1 NZ_CP036188:c129745-129479 [Klebsiella pneumoniae]
MKSDVQLNIRAKEAQRALIDTAAEILHKSRTDFILEMACQAAENVILDRRMFNLNDEQYAEFIDMLDAPVTANPAMEKLL
ARKPQWAK

Nucleotide

• Length: 267 bp

>AT119310 NZ_CP036188:c129745-129479 [Klebsiella pneumoniae]
ATGAAATCTGATGTTCAGCTCAACATCCGGGCGAAAGAAGCCCAGCGGGCTCTGATTGATACTGCCGCTGAGATTCTCCA
CAAATCACGCACTGATTTTATACTGGAGATGGCCTGTCAGGCGGCGGAAAACGTCATTCTTGACCGCCGTATGTTCAATC
TCAATGATGAACAGTACGCCGAGTTTATCGACATGCTGGATGCGCCGGTCACCGCTAACCCGGCGATGGAGAAACTCCTG
GCGAGAAAACCGCAGTGGGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A331KSM6