Detailed information of TA system TA119309

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 105208..105851
• Replicon: plasmid pIncFIBK
• Accession: NZ_CP036188
• Organism: Klebsiella pneumoniae strain BA1559

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: EX352_RS27395
• Protein ID: WP_014386165.1
• Coordinates: 105208..105624 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: EX352_RS27400
• Protein ID: WP_032408893.1
• Coordinates: 105621..105851 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EX352_RS27370	101828..102259	+	432	WP_014386161.1	conjugation system SOS inhibitor PsiB	-
EX352_RS27375	102256..102984	+	729	WP_014386162.1	plasmid SOS inhibition protein A	-
EX352_RS27380	102981..103307	+	327	WP_004152639.1	hypothetical protein	-
EX352_RS27385	103363..103737	+	375	WP_032408891.1	hypothetical protein	-
EX352_RS27390	103917..104885	-	969	WP_074422984.1	IS5 family transposase	-
EX352_RS27395	105208..105624	-	417	WP_014386165.1	PIN domain-containing protein	Toxin
EX352_RS27400	105621..105851	-	231	WP_032408893.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
EX352_RS27410	106171..106605	+	435	WP_000103648.1	RidA family protein	-
EX352_RS27415	106610..107284	+	675	WP_014386166.1	DUF1028 domain-containing protein	-
EX352_RS27420	107300..108457	+	1158	WP_032408895.1	acetylornithine deacetylase	-
EX352_RS27425	108628..109776	+	1149	WP_014386168.1	ABC transporter ATP-binding protein	-
EX352_RS27430	109783..110778	+	996	WP_000611945.1	NAD(P)H-quinone oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(A) / aph(3')-Ia / mph(A) / sul1 / qacE / aadA2 / dfrA12 / blaTEM-1B / blaCTX-M-15 / aac(6')-Ib-cr / blaOXA-1	-	1..251327	251327
	-	flank	IS/Tn	-	-	103917..104885	968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-130)

Antitoxin

• SpoVT-AbrB domain (7-55)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15127.44 Da
• Isoelectric Point: 7.8841

>T119309 WP_014386165.1 NZ_CP036188:c105624-105208 [Klebsiella pneumoniae]
MTKTYMLDTNICSYIMREQPEAVLKRLEQAVLRRHRIVVSAITYAEMRFGAIGKRASPRHVQLVDAFCARLDAILAWDRA
AVDATTEVKATLAAAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVAGLTFEDWVQ

Nucleotide

• Length: 417 bp

>T119309 NZ_CP036188:c105624-105208 [Klebsiella pneumoniae]
ATGACCAAAACCTACATGCTGGATACTAATATCTGCTCTTATATCATGCGCGAACAACCGGAAGCGGTGCTGAAACGCCT
AGAGCAAGCGGTGTTGCGCCGTCACCGGATAGTGGTGTCAGCCATCACCTATGCCGAAATGCGTTTTGGTGCCATCGGTA
AAAGGGCTTCGCCCCGTCATGTGCAGCTGGTGGACGCATTCTGTGCGCGCCTTGATGCCATACTGGCTTGGGATCGGGCC
GCAGTTGACGCTACAACAGAGGTTAAGGCCACGCTAGCAGCTGCCGGCACGCCAATCGGCCCAAACGACACAGCCATTGC
TGGACATGCCATTGCCGCTGGCGCCATCCTGGTGACGAATAATACGCGTGAATTTGAGCGAGTCGCTGGTCTGACGTTTG
AAGACTGGGTGCAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8854.89 Da
• Isoelectric Point: 4.5112

>AT119309 WP_032408893.1 NZ_CP036188:c105851-105621 [Klebsiella pneumoniae]
MRTVSVFRNGNNRAIRLPRDLDFDGVNELEIIREGDTLILRPVKPSWSSFRQEEKADADFMAERENVVSDEGRFEL

Nucleotide

• Length: 231 bp

>AT119309 NZ_CP036188:c105851-105621 [Klebsiella pneumoniae]
ATGCGAACTGTATCTGTATTCAGGAACGGTAATAACCGGGCCATCCGTCTGCCCCGCGATCTGGACTTTGACGGCGTGAA
CGAACTGGAAATCATCCGCGAGGGCGATACCCTCATCCTGCGGCCGGTAAAACCCAGTTGGAGTTCGTTCCGCCAGGAGG
AAAAAGCTGACGCAGATTTTATGGCGGAGCGTGAAAATGTTGTTAGTGACGAGGGACGTTTTGAGCTATGA