Detailed information of TA system TA119308

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 28697..29433
• Replicon: plasmid pIncFIBK
• Accession: NZ_CP036188
• Organism: Klebsiella pneumoniae strain BA1559

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: EX352_RS26955
• Protein ID: WP_003026803.1
• Coordinates: 28697..29179 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: EX352_RS26960
• Protein ID: WP_003026799.1
• Coordinates: 29167..29433 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EX352_RS26940	24335..25297	+	963	WP_004152113.1	M48 family metalloprotease	-
EX352_RS26945	25385..26639	+	1255	Protein_29	IS3 family transposase	-
EX352_RS26950	27139..28485	-	1347	WP_020314316.1	ISNCY family transposase	-
EX352_RS26955	28697..29179	-	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
EX352_RS26960	29167..29433	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
EX352_RS26965	29609..29863	-	255	WP_004152108.1	hypothetical protein	-
EX352_RS26970	29939..30192	-	254	Protein_34	hypothetical protein	-
EX352_RS26975	30241..30444	-	204	WP_004152106.1	hemolysin expression modulator Hha	-
EX352_RS26980	30477..30844	-	368	Protein_36	hypothetical protein	-
EX352_RS26985	30889..31383	-	495	WP_004152104.1	DNA-binding protein	-
EX352_RS26990	31414..31989	-	576	WP_004152103.1	hypothetical protein	-
EX352_RS26995	31977..32246	-	270	WP_004152102.1	hypothetical protein	-
EX352_RS27000	32604..32954	+	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
EX352_RS27005	33004..33366	+	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(A) / aph(3')-Ia / mph(A) / sul1 / qacE / aadA2 / dfrA12 / blaTEM-1B / blaCTX-M-15 / aac(6')-Ib-cr / blaOXA-1	-	1..251327	251327

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T119308 WP_003026803.1 NZ_CP036188:c29179-28697 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T119308 NZ_CP036188:c29179-28697 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT119308 WP_003026799.1 NZ_CP036188:c29433-29167 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT119308 NZ_CP036188:c29433-29167 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66