Detailed information of TA system TA119290

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 6117..6952
• Replicon: chromosome
• Accession: NZ_CP036187
• Organism: Klebsiella pneumoniae strain BA1559

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A5E2RWI0
• Locus tag: EX352_RS00055
• Protein ID: WP_001559913.1
• Coordinates: 6117..6494 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A5E3EVB5
• Locus tag: EX352_RS00060
• Protein ID: WP_032408847.1
• Coordinates: 6584..6952 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EX352_RS00015	1383..1649	+	267	WP_004178415.1	hypothetical protein	-
EX352_RS00020	1649..2321	+	673	Protein_3	DUF4400 domain-containing protein	-
EX352_RS00025	2332..3216	+	885	WP_104013999.1	RES domain-containing protein	-
EX352_RS00030	3415..3603	-	189	Protein_5	transposase	-
EX352_RS00035	3620..4731	-	1112	Protein_6	IS3 family transposase	-
EX352_RS28370	5178..5330	-	153	WP_164713811.1	hypothetical protein	-
EX352_RS00045	5415..5612	-	198	WP_032408848.1	DUF957 domain-containing protein	-
EX352_RS00050	5632..6120	-	489	WP_001559914.1	hypothetical protein	-
EX352_RS00055	6117..6494	-	378	WP_001559913.1	TA system toxin CbtA family protein	Toxin
EX352_RS00060	6584..6952	-	369	WP_032408847.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EX352_RS00065	7129..7350	-	222	WP_000692300.1	DUF987 domain-containing protein	-
EX352_RS00070	7419..7895	-	477	WP_032408846.1	RadC family protein	-
EX352_RS00075	7910..8395	-	486	WP_032408845.1	antirestriction protein	-
EX352_RS00080	8487..9305	-	819	WP_001559911.1	DUF945 domain-containing protein	-
EX352_RS00090	9405..9638	-	234	WP_001119729.1	DUF905 family protein	-
EX352_RS00095	9717..10172	-	456	WP_000581504.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14148.10 Da
• Isoelectric Point: 7.8276

>T119290 WP_001559913.1 NZ_CP036187:c6494-6117 [Klebsiella pneumoniae]
MKTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T119290 NZ_CP036187:c6494-6117 [Klebsiella pneumoniae]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTGCTGGACCAGCATTACGGCCTTACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACTCGCGA
CAATTACAGAACGGTAAATAACATTACCCTAGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13682.62 Da
• Isoelectric Point: 7.8398

>AT119290 WP_032408847.1 NZ_CP036187:c6952-6584 [Klebsiella pneumoniae]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVMLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT119290 NZ_CP036187:c6952-6584 [Klebsiella pneumoniae]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGTGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCATGCTGTATGCGAAAGGTCTGACCTGCAAAGCTGACACCCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A5E2RWI0

Antitoxin

• AlphaFold DB: A0A5E3EVB5