Detailed information of TA system TA119289

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3936734..3937368
• Replicon: chromosome
• Accession: NZ_CP036186
• Organism: Pseudomonas stutzeri strain ATCC 14405

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: Pstu14405_RS18235
• Protein ID: WP_003285519.1
• Coordinates: 3936734..3937138 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: L1LS63
• Locus tag: Pstu14405_RS18240
• Protein ID: WP_003285520.1
• Coordinates: 3937138..3937368 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Pstu14405_RS18210	3932342..3933184	-	843	WP_003285512.1	indole-3-glycerol phosphate synthase TrpC	-
Pstu14405_RS18215	3933181..3934227	-	1047	WP_003285513.1	anthranilate phosphoribosyltransferase	-
Pstu14405_RS18220	3934227..3934820	-	594	WP_003285514.1	aminodeoxychorismate/anthranilate synthase component II	-
Pstu14405_RS18225	3935287..3935619	+	333	WP_036992178.1	hypothetical protein	-
Pstu14405_RS18230	3935679..3936647	+	969	WP_003285518.1	DUF932 domain-containing protein	-
Pstu14405_RS18235	3936734..3937138	-	405	WP_003285519.1	tRNA(fMet)-specific endonuclease VapC	Toxin
Pstu14405_RS18240	3937138..3937368	-	231	WP_003285520.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
Pstu14405_RS18245	3937525..3938529	+	1005	WP_003285521.1	YqaJ viral recombinase family protein	-
Pstu14405_RS18250	3938619..3939515	+	897	WP_003285522.1	hypothetical protein	-
Pstu14405_RS18255	3939557..3939745	+	189	Protein_3578	DNA repair protein RadC	-
Pstu14405_RS18260	3939825..3940832	+	1008	WP_003285525.1	methyl-accepting chemotaxis protein	-
Pstu14405_RS18265	3940963..3941106	-	144	Protein_3580	IS5/IS1182 family transposase	-
Pstu14405_RS18270	3941208..3941525	+	318	WP_003282986.1	IS66 family insertion sequence hypothetical protein	-
Pstu14405_RS18275	3941522..3941857	+	336	WP_003282984.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-35)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14940.14 Da
• Isoelectric Point: 7.2774

>T119289 WP_003285519.1 NZ_CP036186:c3937138-3936734 [Pseudomonas stutzeri]
MLKYMLDTNICIFTIKNRPEQVREAFKRHSGQLSISTVTLMELIYGAEKSANPERNLADVEGFAARLEVLSYDAQAAAHS
GQLRAELARIGKPIGPYDQMIAGHARAQGLILVTNNLREFERVPGLRVEDWVSA

Nucleotide

• Length: 405 bp

>T119289 NZ_CP036186:c3937138-3936734 [Pseudomonas stutzeri]
ATGCTGAAGTACATGCTTGATACCAACATCTGCATTTTCACGATCAAGAATCGCCCTGAGCAGGTGCGGGAAGCCTTCAA
GCGCCATTCAGGGCAGCTGAGCATTAGCACTGTCACCCTGATGGAGCTTATTTACGGCGCCGAGAAGTCCGCTAATCCCG
AGCGTAATCTGGCGGACGTTGAAGGTTTCGCTGCGCGCCTGGAAGTACTGTCTTACGACGCTCAGGCAGCGGCTCATTCC
GGGCAATTGCGAGCTGAGTTGGCTCGTATCGGCAAGCCTATTGGCCCGTATGATCAGATGATTGCAGGACACGCACGTGC
CCAGGGACTGATTCTGGTCACGAATAATCTTCGTGAATTCGAGCGAGTTCCAGGTTTGCGCGTCGAGGATTGGGTGAGCG
CCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8432.41 Da
• Isoelectric Point: 4.2683

>AT119289 WP_003285520.1 NZ_CP036186:c3937368-3937138 [Pseudomonas stutzeri]
MEQGAVFQSNRSQAVRLPKAVALPEDVKRVDVVVVGRARIITPAGEAWDVWFDGEGVTADYMADREQPADQERDAF

Nucleotide

• Length: 231 bp

>AT119289 NZ_CP036186:c3937368-3937138 [Pseudomonas stutzeri]
ATGGAACAAGGTGCAGTTTTTCAGAGCAACCGCAGTCAAGCGGTGCGTTTGCCCAAGGCGGTTGCGCTGCCTGAGGATGT
GAAGCGGGTGGATGTAGTGGTAGTTGGTCGAGCCCGAATCATCACGCCGGCAGGCGAGGCCTGGGACGTCTGGTTCGATG
GCGAAGGTGTGACCGCTGACTACATGGCGGATCGCGAGCAGCCTGCAGATCAAGAGCGGGATGCGTTCTGA

Structures

Antitoxin

• AlphaFold DB: L1LS63