Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 157420..157913 Replicon plasmid pCTXM199_025970
Accession NZ_CP036178
Organism Escherichia coli strain WCHEC025970

Toxin (Protein)


Gene name TsxA Uniprot ID U9Y9W8
Locus tag EYD11_RS24165 Protein ID WP_001230709.1
Coordinates 157420..157674 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID U9Y4R8
Locus tag EYD11_RS24170 Protein ID WP_000151456.1
Coordinates 157671..157913 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EYD11_RS24125 152553..152975 - 423 WP_000025803.1 hypothetical protein -
EYD11_RS24130 153009..153401 - 393 WP_000843244.1 hypothetical protein -
EYD11_RS24135 153426..154175 - 750 WP_000720274.1 thioredoxin fold domain-containing protein -
EYD11_RS24140 154323..154862 + 540 WP_000494967.1 lytic transglycosylase domain-containing protein -
EYD11_RS24145 154993..155511 + 519 WP_015059500.1 hypothetical protein -
EYD11_RS24150 155619..156560 - 942 WP_001129136.1 HNH endonuclease -
EYD11_RS24155 156570..156833 - 264 Protein_160 HNH endonuclease -
EYD11_RS24160 156881..157423 + 543 WP_001083904.1 hypothetical protein -
EYD11_RS24165 157420..157674 + 255 WP_001230709.1 hypothetical protein Toxin
EYD11_RS24170 157671..157913 + 243 WP_000151456.1 hypothetical protein Antitoxin
EYD11_RS24175 158050..158382 + 333 WP_001393253.1 WbuC family cupin fold metalloprotein -
EYD11_RS24180 158429..159304 - 876 WP_087587947.1 class A extended-spectrum beta-lactamase CTX-M-199 -
EYD11_RS24185 159557..160819 - 1263 WP_000608644.1 IS1380-like element ISEc9 family transposase -
EYD11_RS24190 161305..162285 - 981 WP_000019441.1 IS5-like element ISKpn26 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / blaTEM-1B / oqxA / oqxB / mph(A) / sul1 / qacE / aadA16 / dfrA27 / ARR-3 / blaCTX-M-199 - 1..238258 238258


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

SpoVT-AbrB domain

(6-49)

Antitoxin

mRNA interferase PemK-like

(5-104)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9942.47 Da        Isoelectric Point: 10.1206

>T119279 WP_001230709.1 NZ_CP036178:157420-157674 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Download         Length: 255 bp

>T119279 NZ_CP036178:157420-157674 [Escherichia coli]
ATGAGAAAAAATAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9136.45 Da        Isoelectric Point: 7.2775

>AT119279 WP_000151456.1 NZ_CP036178:157671-157913 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Download         Length: 243 bp

>AT119279 NZ_CP036178:157671-157913 [Escherichia coli]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGAAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G9H8F0

Antitoxin

Source ID Structure
AlphaFold DB A0A3G9GNF5

References