Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 40600..41336 Replicon plasmid p1_090001
Accession NZ_CP036176
Organism Klebsiella huaxiensis strain WCHKl090001

Toxin (Protein)


Gene name tacT Uniprot ID A0A2J5Q928
Locus tag DA718_RS29795 Protein ID WP_004098919.1
Coordinates 40854..41336 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A2J5Q9C0
Locus tag DA718_RS29790 Protein ID WP_004098921.1
Coordinates 40600..40866 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DA718_RS29745 35723..36301 - 579 WP_009654302.1 hypothetical protein -
DA718_RS29750 36591..36854 + 264 WP_009310051.1 hypothetical protein -
DA718_RS29755 36851..37417 + 567 WP_009654312.1 hypothetical protein -
DA718_RS29760 37448..37942 + 495 WP_013023776.1 hypothetical protein -
DA718_RS29765 37986..38354 + 369 WP_042946273.1 hypothetical protein -
DA718_RS29770 38385..38588 + 204 WP_004098931.1 hemolysin expression modulator Hha -
DA718_RS29775 38637..38894 + 258 WP_004098928.1 hypothetical protein -
DA718_RS29780 39111..40091 + 981 WP_016947617.1 IS5-like element ISKpn26 family transposase -
DA718_RS29785 40215..40424 + 210 WP_104897472.1 hypothetical protein -
DA718_RS29790 40600..40866 + 267 WP_004098921.1 DUF1778 domain-containing protein Antitoxin
DA718_RS29795 40854..41336 + 483 WP_004098919.1 GNAT family N-acetyltransferase Toxin
DA718_RS29800 41545..42891 + 1347 WP_077255298.1 ISNCY family transposase -
DA718_RS29805 42950..43741 + 792 WP_112217421.1 tetratricopeptide repeat protein -
DA718_RS29810 43782..44564 - 783 Protein_45 hypothetical protein -
DA718_RS29815 44554..44904 + 351 Protein_46 transposase -
DA718_RS29820 44935..45273 + 339 Protein_47 IS5/IS1182 family transposase -
DA718_RS29825 45319..45510 - 192 WP_127074617.1 hypothetical protein -
DA718_RS30555 45507..45668 - 162 WP_003032086.1 hypothetical protein -
DA718_RS29830 45740..46191 - 452 Protein_50 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..117221 117221
- inside IScluster/Tn - - 39111..63149 24038


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17335.06 Da        Isoelectric Point: 10.0704

>T119253 WP_004098919.1 NZ_CP036176:40854-41336 [Klebsiella huaxiensis]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T119253 NZ_CP036176:40854-41336 [Klebsiella huaxiensis]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGAAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCTACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGTGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGCGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10105.49 Da        Isoelectric Point: 4.5141

>AT119253 WP_004098921.1 NZ_CP036176:40600-40866 [Klebsiella huaxiensis]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEIACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT119253 NZ_CP036176:40600-40866 [Klebsiella huaxiensis]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTAGAGATTGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGATTCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q928

Antitoxin

Source ID Structure
AlphaFold DB A0A2J5Q9C0

References