Detailed information of TA system TA119251

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 5663995..5664748
• Replicon: chromosome
• Accession: NZ_CP036175
• Organism: Klebsiella huaxiensis strain WCHKl090001

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: R8WN26
• Locus tag: DA718_RS27065
• Protein ID: WP_016154457.1
• Coordinates: 5664371..5664748 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: DA718_RS27060
• Protein ID: WP_162838704.1
• Coordinates: 5663995..5664321 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DA718_RS27000	5659053..5659319	+	267	WP_009652486.1	hypothetical protein	-
DA718_RS27005	5659332..5659643	+	312	WP_009652410.1	hypothetical protein	-
DA718_RS27010	5659690..5660160	+	471	WP_009652431.1	hypothetical protein	-
DA718_RS27015	5660209..5660721	+	513	WP_009652395.1	DUF4234 domain-containing protein	-
DA718_RS27020	5660804..5661025	+	222	WP_009652443.1	hypothetical protein	-
DA718_RS27025	5661081..5661314	+	234	WP_009652474.1	DUF905 domain-containing protein	-
DA718_RS27030	5661418..5662236	+	819	WP_009652424.1	DUF945 domain-containing protein	-
DA718_RS27035	5662236..5662490	+	255	WP_009652519.1	hypothetical protein	-
DA718_RS27040	5662519..5662980	+	462	WP_045890390.1	antirestriction protein	-
DA718_RS27045	5662991..5663209	+	219	WP_009652503.1	hypothetical protein	-
DA718_RS27050	5663221..5663703	+	483	WP_016154455.1	DNA repair protein RadC	-
DA718_RS27055	5663717..5663938	+	222	WP_009652392.1	DUF987 domain-containing protein	-
DA718_RS27060	5663995..5664321	+	327	WP_162838704.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
DA718_RS27065	5664371..5664748	+	378	WP_016154457.1	TA system toxin CbtA family protein	Toxin
DA718_RS27070	5664745..5665236	+	492	WP_009652438.1	hypothetical protein	-
DA718_RS27075	5665266..5665469	+	204	WP_009652505.1	DUF957 domain-containing protein	-
DA718_RS27080	5665550..5666395	+	846	Protein_5226	DUF4942 domain-containing protein	-
DA718_RS27085	5666407..5666643	+	237	WP_016154458.1	hypothetical protein	-
DA718_RS27090	5666682..5667269	-	588	WP_112216313.1	NAD(P)H-dependent oxidoreductase	-
DA718_RS27095	5667384..5667980	+	597	WP_112216312.1	TetR/AcrR family transcriptional regulator	-
DA718_RS27100	5668066..5668854	-	789	WP_112216311.1	DsbA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14168.19 Da
• Isoelectric Point: 9.5224

>T119251 WP_016154457.1 NZ_CP036175:5664371-5664748 [Klebsiella huaxiensis]
MQTQPVPPKREVSPRPSPVAIWQRLLSHLLDRHYGLTLNDTPFGKDGVIQEHIDAGISLCDAVNFIVEKYDLVRTDRRGF
NAETQSPLLTSIDILRARKATGLMTRHSYRAVTDITTGKYREVQP

Nucleotide

• Length: 378 bp

>T119251 NZ_CP036175:5664371-5664748 [Klebsiella huaxiensis]
ATGCAAACACAACCTGTTCCTCCGAAACGGGAGGTTTCGCCACGCCCGTCACCCGTGGCGATCTGGCAGCGCCTCCTGAG
TCACCTGCTGGACCGTCACTACGGTCTGACGCTCAACGACACGCCATTTGGCAAAGATGGTGTGATCCAAGAGCATATCG
ATGCCGGTATCTCACTGTGCGACGCGGTGAATTTCATCGTGGAGAAATACGACCTGGTACGCACTGATCGTCGCGGTTTT
AACGCGGAAACTCAGTCGCCGCTTCTCACCAGTATCGATATTCTCCGCGCCCGCAAAGCCACCGGGCTGATGACCCGTCA
TAGCTACAGGGCCGTTACCGACATCACCACCGGCAAATACCGTGAGGTGCAACCATGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12079.85 Da
• Isoelectric Point: 6.6283

>AT119251 WP_162838704.1 NZ_CP036175:5663995-5664321 [Klebsiella huaxiensis]
IAAPWWGLKRDITPCFGARLVQEGNRLHYLNDRASITGTFSDADLRHLDQAFPLLLKQLELMLLSGELNPRHQHCVTLYA
KGLICEADSLGSHGYVYLAIYPTPATTE

Nucleotide

• Length: 327 bp

>AT119251 NZ_CP036175:5663995-5664321 [Klebsiella huaxiensis]
ATCGCCGCCCCTTGGTGGGGGCTTAAACGCGATATCACCCCCTGTTTTGGCGCACGTCTGGTGCAGGAGGGCAACCGGCT
GCATTACCTGAACGACCGCGCCAGCATCACCGGCACCTTCAGCGACGCGGATTTACGCCATCTCGACCAGGCATTCCCGC
TACTGCTGAAGCAACTGGAGCTGATGCTGCTTTCCGGTGAGCTGAATCCCCGTCATCAGCACTGCGTCACGCTGTATGCA
AAAGGGCTGATATGCGAGGCCGACAGCCTCGGCTCGCACGGGTACGTCTATCTCGCAATTTACCCGACACCTGCAACAAC
AGAATAA

Structures

Toxin

• AlphaFold DB: R8WN26