Detailed information of TA system TA119232

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2465252..2465817
• Replicon: chromosome
• Accession: NZ_CP036164
• Organism: Janibacter limosus strain P3-3-X1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EXU32_RS11675
• Protein ID: WP_130630066.1
• Coordinates: 2465252..2465623 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: EXU32_RS11680
• Protein ID: WP_130630067.1
• Coordinates: 2465620..2465817 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXU32_RS11645	2460859..2461389	-	531	WP_130630061.1	DinB family protein	-
EXU32_RS11650	2461432..2461974	-	543	WP_130631174.1	methyltransferase domain-containing protein	-
EXU32_RS11655	2462021..2462464	-	444	WP_130630062.1	SRPBCC family protein	-
EXU32_RS11660	2462530..2463459	-	930	WP_130630063.1	NADP-dependent oxidoreductase	-
EXU32_RS11665	2463642..2464004	-	363	WP_130630064.1	alkylphosphonate utilization protein	-
EXU32_RS11670	2464069..2465199	+	1131	WP_130630065.1	PLP-dependent cysteine synthase family protein	-
EXU32_RS11675	2465252..2465623	-	372	WP_130630066.1	type II toxin-antitoxin system VapC family toxin	Toxin
EXU32_RS11680	2465620..2465817	-	198	WP_130630067.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
EXU32_RS11685	2465922..2466437	-	516	WP_130630068.1	DUF1877 family protein	-
EXU32_RS11690	2466648..2467862	+	1215	WP_130630069.1	N(5)-(carboxyethyl)ornithine synthase	-
EXU32_RS11695	2467786..2468571	-	786	WP_130630070.1	hypothetical protein	-
EXU32_RS11700	2468622..2469194	+	573	Protein_2302	histone deacetylase	-
EXU32_RS11705	2469398..2469718	-	321	WP_130630071.1	HigA family addiction module antidote protein	-
EXU32_RS11710	2469693..2469974	-	282	WP_130630072.1	type II toxin-antitoxin system RelE/ParE family toxin	-
EXU32_RS11715	2470125..2470460	-	336	WP_130630073.1	DUF86 domain-containing protein	-
EXU32_RS11720	2470457..2470786	-	330	WP_130630074.1	nucleotidyltransferase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-43)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13838.96 Da
• Isoelectric Point: 6.4868

>T119232 WP_130630066.1 NZ_CP036164:c2465623-2465252 [Janibacter limosus]
VILVDTSIWIDHLRAEEPELVARLQDDAVGCHPYVIEELALGSIRERDRVVGLLADLHTFPVVTHRELLELVDRRRLWGR
GLSAVDTHLLGSVALVPGGRLWTRDRRLLAAARDVGVRCHDTL

Nucleotide

• Length: 372 bp

>T119232 NZ_CP036164:c2465623-2465252 [Janibacter limosus]
GTGATCCTGGTCGACACCTCGATCTGGATCGACCACCTCCGTGCGGAGGAGCCCGAGCTCGTCGCGCGCCTGCAGGACGA
CGCGGTGGGTTGCCACCCCTACGTCATCGAGGAGCTGGCTCTGGGGTCCATCCGAGAGCGTGACCGAGTCGTGGGCCTGC
TCGCTGACCTGCACACGTTCCCTGTCGTGACGCACCGCGAGCTCCTCGAGCTGGTGGACAGACGCCGCCTCTGGGGGCGT
GGTCTCAGCGCTGTCGACACGCACCTGCTCGGGTCGGTCGCGCTGGTCCCCGGTGGGCGGCTGTGGACTCGCGACCGGCG
TCTGCTCGCCGCCGCCCGGGATGTCGGGGTCCGCTGTCACGACACCCTGTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7081.14 Da
• Isoelectric Point: 7.0133

>AT119232 WP_130630067.1 NZ_CP036164:c2465817-2465620 [Janibacter limosus]
MRTTVTIDDELMARATELTGIVERSVLLREGLETLIRVESARRLIALGGADPHATAAPRRRGAPE

Nucleotide

• Length: 198 bp

>AT119232 NZ_CP036164:c2465817-2465620 [Janibacter limosus]
ATGCGCACGACGGTGACGATCGACGATGAGCTCATGGCCAGGGCGACGGAGCTGACGGGGATCGTGGAGCGTTCGGTCCT
GCTGCGCGAGGGACTCGAGACGCTCATCCGGGTCGAGAGCGCTCGCCGACTGATTGCCCTCGGCGGTGCAGACCCCCATG
CCACGGCGGCCCCACGGCGTCGAGGCGCGCCGGAGTGA