Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /SpoIISA(toxin)
Location 2368844..2369822 Replicon chromosome
Accession NZ_CP036145
Organism Bacillus mycoides strain JAS94/5

Toxin (Protein)


Gene name spoIISA Uniprot ID J8I6P2
Locus tag EXW57_RS12095 Protein ID WP_002017051.1
Coordinates 2368844..2369584 (+) Length 247 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID R8HWP4
Locus tag EXW57_RS12100 Protein ID WP_000588712.1
Coordinates 2369697..2369822 (+) Length 42 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXW57_RS12065 (EXW57_12360) 2363947..2364708 + 762 WP_002127420.1 ABC transporter ATP-binding protein -
EXW57_RS12070 (EXW57_12365) 2364814..2365524 + 711 WP_002127421.1 class I SAM-dependent methyltransferase -
EXW57_RS12075 (EXW57_12370) 2365643..2366056 + 414 WP_002012925.1 VOC family protein -
EXW57_RS12080 (EXW57_12375) 2366232..2366864 - 633 WP_002017047.1 type 1 glutamine amidotransferase family protein -
EXW57_RS12085 (EXW57_12380) 2367138..2367614 + 477 WP_002017049.1 MarR family winged helix-turn-helix transcriptional regulator -
EXW57_RS12090 (EXW57_12385) 2367768..2368505 + 738 WP_215566699.1 2,3-diphosphoglycerate-dependent phosphoglycerate mutase -
EXW57_RS12095 (EXW57_12390) 2368844..2369584 + 741 WP_002017051.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
EXW57_RS12100 (EXW57_12395) 2369697..2369822 + 126 WP_000588712.1 hypothetical protein Antitoxin
EXW57_RS12105 2369898..2370074 + 177 WP_003189253.1 stage II sporulation protein SB -
EXW57_RS12110 (EXW57_12400) 2370095..2370484 - 390 WP_033733970.1 YxeA family protein -
EXW57_RS12115 (EXW57_12405) 2370736..2372205 + 1470 WP_016126945.1 aminoacyl-histidine dipeptidase -
EXW57_RS12120 2372565..2372714 + 150 WP_002069316.1 hypothetical protein -
EXW57_RS12125 (EXW57_12410) 2373428..2374087 + 660 WP_003189261.1 CatB-related O-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(10-244)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 247 a.a.        Molecular weight: 28512.20 Da        Isoelectric Point: 8.3034

>T119227 WP_002017051.1 NZ_CP036145:2368844-2369584 [Bacillus mycoides]
MTISNIRIGLFILAIVFIVLVFFYWRNEELYEEKKQRIRKTWYGLFITSVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIA
FILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPTAFGTMEWHTEEEYTKSLNTFLDSYGEKIGAK
IVVFEAVKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIH
HSYFSK

Download         Length: 741 bp

>T119227 NZ_CP036145:2368844-2369584 [Bacillus mycoides]
TTGACGATCTCTAACATTCGAATTGGCTTATTTATTTTAGCAATCGTCTTTATTGTTCTTGTTTTCTTTTATTGGAGAAA
TGAAGAGTTGTACGAGGAGAAGAAACAACGAATTCGAAAAACGTGGTACGGTTTGTTCATAACATCAGTCACAGTTTATT
TCATGATAAAAGGAATAGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCGATGGTAATTTTCGTTGATATTGCA
TTCATTTTGACACCAAATATTTCAGAAATATGGGGCGCAAAATTCAGCGATATTGGAAAGACGGTCCAATCAATTAAAAG
GTCATTAATTGCTTCAAAAGCAAGAGGAGAAATATATACGACAATTATTCAAAATGTGAATCCAACAGCCTTTGGGACAA
TGGAATGGCATACGGAAGAGGAATATACAAAAAGCTTAAACACATTTTTAGATTCATATGGGGAAAAGATTGGTGCGAAA
ATTGTTGTATTTGAAGCGGTGAAGGAATTAAATACAAACTTTCGTGGTATCCGCTCTCAATTTAGTATTATTGTTCCATT
AGAACACATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTTAGTG
ATGTTTTCATTGTTATTGATGGGAAGAAAAATAACCTTCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATTCAT
CATAGTTATTTTAGTAAATAA

Antitoxin


Download         Length: 42 a.a.        Molecular weight: 4938.34 Da        Isoelectric Point: 3.9150

>AT119227 WP_000588712.1 NZ_CP036145:2369697-2369822 [Bacillus mycoides]
VIKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRK

Download         Length: 126 bp

>AT119227 NZ_CP036145:2369697-2369822 [Bacillus mycoides]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACAGATTTCTCTTTAATAAAAGGAGATAC
TTTGCAGCAAGTAGAAGAGTTAGAAGAAGAATTAAAAAGAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB J8I6P2

Antitoxin

Source ID Structure
AlphaFold DB A0A366GQT1

References