Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /SpoIISA(toxin)
Location 3056573..3057551 Replicon chromosome
Accession NZ_CP036137
Organism Bacillus mycoides strain JAS85/1

Toxin (Protein)


Gene name spoIISA Uniprot ID J8I6P2
Locus tag EXW42_RS15670 Protein ID WP_002017051.1
Coordinates 3056573..3057313 (+) Length 247 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID R8HWP4
Locus tag EXW42_RS15675 Protein ID WP_000588712.1
Coordinates 3057426..3057551 (+) Length 42 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXW42_RS15640 (EXW42_16010) 3051675..3052436 + 762 WP_002127420.1 ABC transporter ATP-binding protein -
EXW42_RS15645 (EXW42_16015) 3052542..3053252 + 711 WP_002127421.1 class I SAM-dependent methyltransferase -
EXW42_RS15650 (EXW42_16020) 3053371..3053784 + 414 WP_002012925.1 VOC family protein -
EXW42_RS15655 (EXW42_16025) 3053961..3054593 - 633 WP_002017047.1 type 1 glutamine amidotransferase family protein -
EXW42_RS15660 (EXW42_16030) 3054867..3055343 + 477 WP_002017049.1 MarR family winged helix-turn-helix transcriptional regulator -
EXW42_RS15665 (EXW42_16035) 3055497..3056234 + 738 WP_002032137.1 2,3-diphosphoglycerate-dependent phosphoglycerate mutase -
EXW42_RS15670 (EXW42_16040) 3056573..3057313 + 741 WP_002017051.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
EXW42_RS15675 (EXW42_16045) 3057426..3057551 + 126 WP_000588712.1 hypothetical protein Antitoxin
EXW42_RS15680 3057627..3057803 + 177 WP_003189253.1 stage II sporulation protein SB -
EXW42_RS15685 (EXW42_16050) 3057824..3058213 - 390 WP_033731043.1 YxeA family protein -
EXW42_RS15690 (EXW42_16055) 3058459..3059928 + 1470 WP_016126945.1 aminoacyl-histidine dipeptidase -
EXW42_RS15695 3060288..3060437 + 150 WP_002069316.1 hypothetical protein -
EXW42_RS15700 (EXW42_16060) 3061151..3061810 + 660 WP_003189261.1 CatB-related O-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(10-244)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 247 a.a.        Molecular weight: 28512.20 Da        Isoelectric Point: 8.3034

>T119225 WP_002017051.1 NZ_CP036137:3056573-3057313 [Bacillus mycoides]
MTISNIRIGLFILAIVFIVLVFFYWRNEELYEEKKQRIRKTWYGLFITSVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIA
FILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPTAFGTMEWHTEEEYTKSLNTFLDSYGEKIGAK
IVVFEAVKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIH
HSYFSK

Download         Length: 741 bp

>T119225 NZ_CP036137:3056573-3057313 [Bacillus mycoides]
TTGACGATCTCTAACATTCGAATTGGCTTATTTATTTTAGCAATCGTCTTTATTGTTCTTGTTTTCTTTTATTGGAGAAA
TGAAGAGTTGTACGAGGAGAAGAAACAACGAATTCGAAAAACGTGGTACGGTTTGTTCATAACATCAGTCACAGTTTATT
TCATGATAAAAGGAATAGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCGATGGTAATTTTCGTTGATATTGCA
TTCATTTTGACACCAAATATTTCAGAAATATGGGGCGCAAAATTCAGCGATATTGGAAAGACGGTCCAATCAATTAAAAG
GTCATTAATTGCTTCAAAAGCAAGAGGAGAAATATATACGACAATTATTCAAAATGTGAATCCAACAGCCTTTGGGACAA
TGGAATGGCATACGGAAGAGGAATATACAAAAAGCTTAAACACATTTTTAGATTCATATGGGGAAAAGATTGGTGCGAAA
ATTGTTGTATTTGAAGCGGTGAAGGAATTAAATACAAACTTTCGTGGTATCCGCTCTCAATTTAGTATTATTGTTCCATT
AGAACACATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTTAGTG
ATGTTTTCATTGTTATTGATGGGAAGAAAAATAACCTTCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATTCAT
CATAGTTATTTTAGTAAATAA

Antitoxin


Download         Length: 42 a.a.        Molecular weight: 4938.34 Da        Isoelectric Point: 3.9150

>AT119225 WP_000588712.1 NZ_CP036137:3057426-3057551 [Bacillus mycoides]
VIKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRK

Download         Length: 126 bp

>AT119225 NZ_CP036137:3057426-3057551 [Bacillus mycoides]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACAGATTTCTCTTTAATAAAAGGAGATAC
TTTGCAGCAAGTAGAAGAGTTAGAAGAAGAATTAAAAAGAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB J8I6P2

Antitoxin

Source ID Structure
AlphaFold DB A0A366GQT1

References