Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 253602..254244 Replicon chromosome
Accession NZ_CP036132
Organism Bacillus mycoides strain JAS823

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag EXW56_RS01410 Protein ID WP_000635965.1
Coordinates 253894..254244 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag EXW56_RS01405 Protein ID WP_000004570.1
Coordinates 253602..253889 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXW56_RS01380 (EXW56_01385) 248762..249724 + 963 WP_215597131.1 UV DNA damage repair endonuclease UvsE -
EXW56_RS01385 (EXW56_01390) 249895..250443 - 549 WP_002113424.1 rhomboid family intramembrane serine protease -
EXW56_RS01390 (EXW56_01395) 250536..250895 + 360 WP_070169775.1 holo-ACP synthase -
EXW56_RS01395 (EXW56_01400) 251052..252002 + 951 WP_002201994.1 outer membrane lipoprotein carrier protein LolA -
EXW56_RS01400 (EXW56_01405) 252120..253289 + 1170 WP_002201993.1 alanine racemase -
EXW56_RS01405 (EXW56_01410) 253602..253889 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
EXW56_RS01410 (EXW56_01415) 253894..254244 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EXW56_RS01415 (EXW56_01420) 254313..256481 + 2169 WP_215597561.1 Tex family protein -
EXW56_RS01420 (EXW56_01425) 256539..256655 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
EXW56_RS01425 (EXW56_01430) 256863..257318 + 456 WP_002201991.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T119222 WP_000635965.1 NZ_CP036132:253894-254244 [Bacillus mycoides]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T119222 NZ_CP036132:253894-254244 [Bacillus mycoides]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCGACTGTGATTGTAGCGGCTATTACTGCGCAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT119222 WP_000004570.1 NZ_CP036132:253602-253889 [Bacillus mycoides]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT119222 NZ_CP036132:253602-253889 [Bacillus mycoides]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References