Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /SpoIISA(toxin)
Location 2378981..2379959 Replicon chromosome
Accession NZ_CP036117
Organism Bacillus mycoides strain JAS481

Toxin (Protein)


Gene name spoIISA Uniprot ID J8I6P2
Locus tag EXW43_RS12680 Protein ID WP_002017051.1
Coordinates 2378981..2379721 (+) Length 247 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID R8HWP4
Locus tag EXW43_RS12685 Protein ID WP_000588712.1
Coordinates 2379834..2379959 (+) Length 42 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXW43_RS12650 (EXW43_12950) 2374083..2374844 + 762 WP_002127420.1 ABC transporter ATP-binding protein -
EXW43_RS12655 (EXW43_12955) 2374950..2375660 + 711 WP_002127421.1 class I SAM-dependent methyltransferase -
EXW43_RS12660 (EXW43_12960) 2375779..2376192 + 414 WP_002012925.1 VOC family protein -
EXW43_RS12665 (EXW43_12965) 2376369..2377001 - 633 WP_002017047.1 type 1 glutamine amidotransferase family protein -
EXW43_RS12670 (EXW43_12970) 2377275..2377751 + 477 WP_002017049.1 MarR family winged helix-turn-helix transcriptional regulator -
EXW43_RS12675 (EXW43_12975) 2377905..2378642 + 738 WP_002032137.1 2,3-diphosphoglycerate-dependent phosphoglycerate mutase -
EXW43_RS12680 (EXW43_12980) 2378981..2379721 + 741 WP_002017051.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
EXW43_RS12685 (EXW43_12985) 2379834..2379959 + 126 WP_000588712.1 hypothetical protein Antitoxin
EXW43_RS12690 2380035..2380211 + 177 WP_003189253.1 stage II sporulation protein SB -
EXW43_RS12695 (EXW43_12990) 2380232..2380621 - 390 WP_033733970.1 YxeA family protein -
EXW43_RS12700 (EXW43_12995) 2380873..2382342 + 1470 WP_003189257.1 aminoacyl-histidine dipeptidase -
EXW43_RS12705 2382702..2382851 + 150 WP_002069316.1 hypothetical protein -
EXW43_RS12710 (EXW43_13000) 2383565..2384224 + 660 WP_003189261.1 CatB-related O-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(10-244)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 247 a.a.        Molecular weight: 28512.20 Da        Isoelectric Point: 8.3034

>T119219 WP_002017051.1 NZ_CP036117:2378981-2379721 [Bacillus mycoides]
MTISNIRIGLFILAIVFIVLVFFYWRNEELYEEKKQRIRKTWYGLFITSVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIA
FILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPTAFGTMEWHTEEEYTKSLNTFLDSYGEKIGAK
IVVFEAVKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIH
HSYFSK

Download         Length: 741 bp

>T119219 NZ_CP036117:2378981-2379721 [Bacillus mycoides]
TTGACGATCTCTAACATTCGAATTGGCTTATTTATTTTAGCAATCGTCTTTATTGTTCTTGTTTTCTTTTATTGGAGAAA
TGAAGAGTTGTACGAGGAGAAGAAACAACGAATTCGAAAAACGTGGTACGGTTTGTTCATAACATCAGTCACAGTTTATT
TCATGATAAAAGGAATAGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCGATGGTAATTTTCGTTGATATTGCA
TTCATTTTGACACCAAATATTTCAGAAATATGGGGCGCAAAATTCAGCGATATTGGAAAGACGGTCCAATCAATTAAAAG
GTCATTAATTGCTTCAAAAGCAAGAGGAGAAATATATACGACAATTATTCAAAATGTGAATCCAACAGCCTTTGGGACAA
TGGAATGGCATACGGAAGAGGAATATACAAAAAGCTTAAACACATTTTTAGATTCATATGGGGAAAAGATTGGTGCGAAA
ATTGTTGTATTTGAAGCGGTGAAGGAATTAAATACAAACTTTCGTGGTATCCGCTCTCAATTTAGTATTATTGTTCCATT
AGAACACATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTTAGTG
ATGTTTTCATTGTTATTGATGGGAAGAAAAATAACCTTCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATTCAT
CATAGTTATTTTAGTAAATAA

Antitoxin


Download         Length: 42 a.a.        Molecular weight: 4938.34 Da        Isoelectric Point: 3.9150

>AT119219 WP_000588712.1 NZ_CP036117:2379834-2379959 [Bacillus mycoides]
VIKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRK

Download         Length: 126 bp

>AT119219 NZ_CP036117:2379834-2379959 [Bacillus mycoides]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACAGATTTCTCTTTAATAAAAGGAGATAC
TTTGCAGCAAGTAGAAGAGTTAGAAGAAGAATTAAAAAGAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB J8I6P2

Antitoxin

Source ID Structure
AlphaFold DB A0A366GQT1

References