Detailed information of TA system TA119214

Bioinformatically predicted

Overview

TA module

• Type: III
• Classification (family/domain): toxIN/ToxN(toxin)
• Location: 39398..40129
• Replicon: plasmid p124
• Accession: NZ_CP036116
• Organism: Bacillus mycoides strain JAS481

Toxin (Protein)

• Gene name: toxN
• Uniprot ID: -
• Locus tag: EXW43_RS00255
• Protein ID: WP_016128092.1
• Coordinates: 39557..40129 (+)
• Length: 191 a.a.

Antitoxin (RNA)

Gene name	toxI
Locus tag	-
Coordinates	39398..39494 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXW43_RS00230 (34625)	34625..34834	+	210	WP_215575811.1	DUF5511 family protein	-
EXW43_RS00235 (34831)	34831..36600	+	1770	Protein_37	ArdC-like ssDNA-binding domain-containing protein	-
EXW43_RS00240 (36664)	36664..37650	+	987	WP_215575812.1	hypothetical protein	-
EXW43_RS00245 (37672)	37672..38223	+	552	Protein_39	metal-dependent hydrolase	-
EXW43_RS00250 (38302)	38302..39327	+	1026	WP_215575813.1	DUF3991 and TOPRIM domain-containing protein	-
- (39398)	39398..39494	+	97	NuclAT_0	-	Antitoxin
- (39398)	39398..39494	+	97	NuclAT_0	-	Antitoxin
- (39398)	39398..39494	+	97	NuclAT_0	-	Antitoxin
- (39398)	39398..39494	+	97	NuclAT_0	-	Antitoxin
EXW43_RS00255 (39557)	39557..40129	+	573	WP_016128092.1	type III toxin-antitoxin system ToxN/AbiQ family toxin	Toxin
EXW43_RS00260 (40517)	40517..42658	+	2142	WP_215575814.1	type IA DNA topoisomerase	-
EXW43_RS00265 (42688)	42688..43131	+	444	WP_215575815.1	CPBP family intramembrane metalloprotease	-
EXW43_RS00270 (43376)	43376..44245	+	870	WP_215575816.1	hypothetical protein	-
EXW43_RS00275 (44555)	44555..44788	-	234	WP_016128097.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..124309	124309

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ToxN/AbiQ toxin (8-171)

Sequences

Toxin

Protein

• Length: 191 a.a.
• Molecular weight: 22390.65 Da
• Isoelectric Point: 9.3730

>T119214 WP_016128092.1 NZ_CP036116:39557-40129 [Bacillus mycoides]
MANKDNPKFYTISTKYIDYLRETDSKVPFNKDEQHSRPYVGVLEKINGHDYFVPLTSRNDKNFNSQVSVKLFDNDNTEKR
IGVLLINNMIPVPEKECKEIDIAEKTAADPKYGNLMLKQYLFLKENMDRVSNKVEKIYKDVSVQGKPSQKQKFIKGVCCD
FPKLEEKCQEYKERDQARRIAYMRQMGRER

Nucleotide

• Length: 573 bp

>T119214 NZ_CP036116:39557-40129 [Bacillus mycoides]
TTGGCTAATAAAGATAATCCTAAATTTTACACTATTTCAACTAAATATATAGACTATCTAAGAGAAACAGATAGTAAAGT
ACCCTTTAATAAAGATGAACAGCATAGCAGACCGTATGTAGGCGTATTGGAAAAAATCAATGGTCATGATTATTTTGTAC
CTTTAACATCACGTAATGATAAAAATTTTAATAGTCAGGTCAGTGTAAAATTATTCGATAACGATAACACTGAGAAGCGA
ATTGGAGTTTTATTAATAAATAACATGATTCCAGTCCCAGAAAAAGAGTGTAAAGAAATTGATATAGCAGAAAAAACGGC
GGCAGACCCTAAGTATGGTAATTTGATGTTAAAGCAGTATTTGTTTTTAAAAGAGAACATGGATAGAGTGTCTAATAAAG
TAGAAAAAATATATAAAGATGTATCTGTACAAGGGAAGCCTTCACAAAAACAGAAATTCATTAAAGGCGTTTGTTGTGAT
TTCCCTAAATTAGAAGAAAAGTGCCAAGAATATAAAGAAAGAGATCAAGCTAGAAGGATAGCATATATGCGCCAAATGGG
GCGTGAGAGATAA

Antitoxin

Nucleotide

• Length: 97 bp

>AT119214 NZ_CP036116:39398-39494 [Bacillus mycoides]
GTAGCCTTACTTTAGTAGGCGCGAAAAAATTGGTGTAACTCTACCGTAGTGAGTACCAAAAAATTGGTGTAGCCTTACCG
TAGTAGGCGCAAAAAAA

Structures

Antitoxin