Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /SpoIISA(toxin)
Location 2437043..2438021 Replicon chromosome
Accession NZ_CP036111
Organism Bacillus toyonensis strain JAS411

Toxin (Protein)


Gene name spoIISA Uniprot ID A0A2B4WYW4
Locus tag EXW25_RS12335 Protein ID WP_000155915.1
Coordinates 2437043..2437783 (+) Length 247 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID J8KE32
Locus tag EXW25_RS12340 Protein ID WP_000588713.1
Coordinates 2437896..2438021 (+) Length 42 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXW25_RS12315 (EXW25_12520) 2432705..2433487 + 783 WP_097954109.1 class I SAM-dependent methyltransferase -
EXW25_RS12320 (EXW25_12525) 2433666..2435342 - 1677 WP_215595959.1 alpha-keto acid decarboxylase family protein -
EXW25_RS12325 (EXW25_12530) 2435469..2435951 + 483 WP_000196695.1 MarR family winged helix-turn-helix transcriptional regulator -
EXW25_RS12330 (EXW25_12535) 2436119..2436856 + 738 WP_097954107.1 2,3-diphosphoglycerate-dependent phosphoglycerate mutase -
EXW25_RS12335 (EXW25_12540) 2437043..2437783 + 741 WP_000155915.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
EXW25_RS12340 (EXW25_12545) 2437896..2438021 + 126 WP_000588713.1 hypothetical protein Antitoxin
EXW25_RS12345 (EXW25_12550) 2438097..2438273 + 177 WP_000808012.1 stage II sporulation protein SB -
EXW25_RS12350 (EXW25_12555) 2438322..2438710 - 389 Protein_2369 YxeA family protein -
EXW25_RS12355 (EXW25_12560) 2438954..2440432 + 1479 WP_097954106.1 beta-Ala-His dipeptidase -
EXW25_RS12360 (EXW25_12565) 2440745..2441554 + 810 WP_097954105.1 papain-like cysteine protease family protein -
EXW25_RS12365 (EXW25_12570) 2441582..2442184 + 603 WP_097954104.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(10-244)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 247 a.a.        Molecular weight: 28416.00 Da        Isoelectric Point: 7.3946

>T119213 WP_000155915.1 NZ_CP036111:2437043-2437783 [Bacillus toyonensis]
MTISNIRIGLFILAIVFVVLVFFYWRNEELYEEKKQRIRKTWYGLFITSVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIS
FILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTNIIQNINPAAFGTMEWHTEEEYTKSLNAFLDSYGEKIGAK
IVVFEAANELNTGFRGIRSQFSITIPIEHIEQLNEQKAVQVENVGIIPAKIMNDVFIVIDGKKNSLQDRDFENVYNLTIH
HSYFSK

Download         Length: 741 bp

>T119213 NZ_CP036111:2437043-2437783 [Bacillus toyonensis]
TTGACGATCTCTAACATTCGAATTGGCTTATTTATTTTAGCAATCGTTTTTGTAGTTCTTGTTTTCTTTTATTGGAGAAA
TGAAGAGTTATATGAAGAGAAAAAGCAGCGAATTCGAAAAACTTGGTATGGATTATTCATAACATCAGTCACAGTTTATT
TCATGATTAAAGGAATCGATTTAACCCTCTGGAAAAACTTATTAATGTTCACTGCGATGGTCATTTTTGTTGATATTTCA
TTTATTTTAACACCTAATATTTCCGAAATATGGGGAGCGAAATTTAGCGATATCGGTAAAACTGTTCAATCCATAAAGCG
GTCATTAATCGCTTCAAAAGCAAGAGGAGAAATATATACGAACATTATCCAAAATATTAATCCAGCAGCATTTGGGACGA
TGGAATGGCATACGGAAGAGGAATATACAAAAAGCTTAAACGCATTTTTAGATTCATACGGGGAAAAAATTGGAGCGAAA
ATCGTTGTATTTGAAGCAGCGAATGAATTAAATACGGGCTTTCGTGGTATTCGTTCTCAATTTAGTATTACAATACCTAT
AGAGCATATAGAGCAATTGAATGAACAAAAAGCAGTGCAAGTAGAAAACGTGGGAATTATACCAGCAAAAATAATGAATG
ATGTTTTTATCGTGATTGATGGGAAGAAGAATAGCCTGCAGGATCGAGATTTTGAAAATGTCTATAATTTAACAATACAT
CATAGTTATTTTAGTAAGTAG

Antitoxin


Download         Length: 42 a.a.        Molecular weight: 4951.43 Da        Isoelectric Point: 4.1079

>AT119213 WP_000588713.1 NZ_CP036111:2437896-2438021 [Bacillus toyonensis]
VIKDYDNDFFKEENEDTTDFSLIKGDTLQQVEKLEEELKRK

Download         Length: 126 bp

>AT119213 NZ_CP036111:2437896-2438021 [Bacillus toyonensis]
GTGATAAAAGATTATGACAATGATTTTTTCAAAGAAGAAAATGAAGATACAACAGATTTCTCTTTAATCAAAGGAGATAC
GTTGCAGCAAGTAGAAAAGTTAGAAGAAGAATTAAAAAGAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2B4WYW4

Antitoxin

Source ID Structure
AlphaFold DB J8KE32

References