119206

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 246921..247563 Replicon chromosome
Accession NZ_CP036102
Organism Bacillus mycoides strain JAS391

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag EXW34_RS01390 Protein ID WP_000635965.1
Coordinates 247213..247563 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag EXW34_RS01385 Protein ID WP_000004570.1
Coordinates 246921..247208 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXW34_RS01360 (EXW34_01365) 242236..243198 + 963 WP_002009966.1 UV DNA damage repair endonuclease UvsE -
EXW34_RS01365 (EXW34_01370) 243191..243763 - 573 WP_002191748.1 rhomboid family intramembrane serine protease -
EXW34_RS01370 (EXW34_01375) 243856..244215 + 360 WP_002009969.1 holo-ACP synthase -
EXW34_RS01375 (EXW34_01380) 244372..245322 + 951 WP_002124820.1 outer membrane lipoprotein carrier protein LolA -
EXW34_RS01380 (EXW34_01385) 245440..246609 + 1170 WP_002124821.1 alanine racemase -
EXW34_RS01385 (EXW34_01390) 246921..247208 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
EXW34_RS01390 (EXW34_01395) 247213..247563 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EXW34_RS01395 (EXW34_01400) 247632..249800 + 2169 WP_002124824.1 Tex family protein -
EXW34_RS01400 (EXW34_01405) 249858..249974 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
EXW34_RS01405 (EXW34_01410) 250185..250640 + 456 WP_016095131.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T119206 WP_000635965.1 NZ_CP036102:247213-247563 [Bacillus mycoides]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T119206 NZ_CP036102:247213-247563 [Bacillus mycoides]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCGACTGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGGGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT119206 WP_000004570.1 NZ_CP036102:246921-247208 [Bacillus mycoides]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT119206 NZ_CP036102:246921-247208 [Bacillus mycoides]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References