119204

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 249206..249848 Replicon chromosome
Accession NZ_CP036101
Organism Bacillus wiedmannii strain JAS28/2

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag EXW48_RS01405 Protein ID WP_000635963.1
Coordinates 249498..249848 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag EXW48_RS01400 Protein ID WP_000004570.1
Coordinates 249206..249493 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXW48_RS01375 (EXW48_01375) 244522..245484 + 963 WP_137051694.1 UV DNA damage repair endonuclease UvsE -
EXW48_RS01380 (EXW48_01380) 245477..246049 - 573 WP_215562510.1 rhomboid family intramembrane serine protease -
EXW48_RS01385 (EXW48_01385) 246142..246501 + 360 WP_000635044.1 holo-ACP synthase -
EXW48_RS01390 (EXW48_01390) 246658..247608 + 951 WP_215563410.1 outer membrane lipoprotein carrier protein LolA -
EXW48_RS01395 (EXW48_01395) 247727..248896 + 1170 WP_215562511.1 alanine racemase -
EXW48_RS01400 (EXW48_01400) 249206..249493 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
EXW48_RS01405 (EXW48_01405) 249498..249848 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EXW48_RS01410 (EXW48_01410) 249917..252085 + 2169 WP_215562512.1 Tex family protein -
EXW48_RS01415 (EXW48_01415) 252143..252259 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
EXW48_RS01420 (EXW48_01420) 252455..252913 + 459 WP_097918246.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T119204 WP_000635963.1 NZ_CP036101:249498-249848 [Bacillus wiedmannii]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T119204 NZ_CP036101:249498-249848 [Bacillus wiedmannii]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CTAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT119204 WP_000004570.1 NZ_CP036101:249206-249493 [Bacillus wiedmannii]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT119204 NZ_CP036101:249206-249493 [Bacillus wiedmannii]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCGGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References