Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 245363..246005 Replicon chromosome
Accession NZ_CP036099
Organism Bacillus mycoides strain JAS23/1

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag EXW47_RS01380 Protein ID WP_000635965.1
Coordinates 245655..246005 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag EXW47_RS01375 Protein ID WP_000004570.1
Coordinates 245363..245650 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXW47_RS01350 (EXW47_01360) 240678..241640 + 963 WP_002009966.1 UV DNA damage repair endonuclease UvsE -
EXW47_RS01355 (EXW47_01365) 241633..242205 - 573 WP_002124818.1 rhomboid family intramembrane serine protease -
EXW47_RS01360 (EXW47_01370) 242298..242657 + 360 WP_002009969.1 holo-ACP synthase -
EXW47_RS01365 (EXW47_01375) 242814..243764 + 951 WP_002091373.1 outer membrane lipoprotein carrier protein LolA -
EXW47_RS01370 (EXW47_01380) 243882..245051 + 1170 WP_002124821.1 alanine racemase -
EXW47_RS01375 (EXW47_01385) 245363..245650 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
EXW47_RS01380 (EXW47_01390) 245655..246005 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EXW47_RS01385 (EXW47_01395) 246074..248242 + 2169 WP_002029442.1 Tex family protein -
EXW47_RS01390 (EXW47_01400) 248300..248416 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
EXW47_RS01395 (EXW47_01405) 248627..249082 + 456 WP_215560863.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T119202 WP_000635965.1 NZ_CP036099:245655-246005 [Bacillus mycoides]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T119202 NZ_CP036099:245655-246005 [Bacillus mycoides]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCGACTGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGGGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT119202 WP_000004570.1 NZ_CP036099:245363-245650 [Bacillus mycoides]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT119202 NZ_CP036099:245363-245650 [Bacillus mycoides]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References