Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 238312..238954 Replicon chromosome
Accession NZ_CP036094
Organism Bacillus toyonensis strain JAS22/1

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag EXW54_RS01355 Protein ID WP_000635963.1
Coordinates 238604..238954 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag EXW54_RS01350 Protein ID WP_000004570.1
Coordinates 238312..238599 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXW54_RS01325 (EXW54_01340) 233639..234601 + 963 WP_098812120.1 UV DNA damage repair endonuclease UvsE -
EXW54_RS01330 (EXW54_01345) 234594..235166 - 573 WP_000908503.1 rhomboid family intramembrane serine protease -
EXW54_RS01335 (EXW54_01350) 235259..235618 + 360 WP_000635049.1 holo-ACP synthase -
EXW54_RS01340 (EXW54_01355) 235775..236725 + 951 WP_002037834.1 outer membrane lipoprotein carrier protein LolA -
EXW54_RS01345 (EXW54_01360) 236843..238012 + 1170 WP_000390621.1 alanine racemase -
EXW54_RS01350 (EXW54_01365) 238312..238599 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
EXW54_RS01355 (EXW54_01370) 238604..238954 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EXW54_RS01360 (EXW54_01375) 239023..241191 + 2169 WP_215575697.1 Tex family protein -
EXW54_RS01365 (EXW54_01380) 241250..241366 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
EXW54_RS01370 (EXW54_01385) 241574..242068 + 495 WP_215575698.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T119200 WP_000635963.1 NZ_CP036094:238604-238954 [Bacillus toyonensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T119200 NZ_CP036094:238604-238954 [Bacillus toyonensis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGATTTGAGAGAGATTCTGTTATTTTGCTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGATTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT119200 WP_000004570.1 NZ_CP036094:238312-238599 [Bacillus toyonensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT119200 NZ_CP036094:238312-238599 [Bacillus toyonensis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACGGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References