119198

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 238340..238982 Replicon chromosome
Accession NZ_CP036090
Organism Bacillus toyonensis strain JAS13/1

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag EXW29_RS01350 Protein ID WP_000635963.1
Coordinates 238632..238982 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag EXW29_RS01345 Protein ID WP_000004570.1
Coordinates 238340..238627 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXW29_RS01320 (EXW29_01335) 233667..234629 + 963 WP_087971138.1 UV DNA damage repair endonuclease UvsE -
EXW29_RS01325 (EXW29_01340) 234622..235194 - 573 WP_097953580.1 rhomboid family intramembrane serine protease -
EXW29_RS01330 (EXW29_01345) 235287..235646 + 360 WP_000583421.1 holo-ACP synthase -
EXW29_RS01335 (EXW29_01350) 235803..236753 + 951 WP_002059179.1 outer membrane lipoprotein carrier protein LolA -
EXW29_RS01340 (EXW29_01355) 236871..238040 + 1170 WP_097953579.1 alanine racemase -
EXW29_RS01345 (EXW29_01360) 238340..238627 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
EXW29_RS01350 (EXW29_01365) 238632..238982 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EXW29_RS01355 (EXW29_01370) 239050..241218 + 2169 WP_142312932.1 Tex family protein -
EXW29_RS01360 (EXW29_01375) 241277..241393 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
EXW29_RS01365 (EXW29_01380) 241601..242059 + 459 WP_000544526.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T119198 WP_000635963.1 NZ_CP036090:238632-238982 [Bacillus toyonensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T119198 NZ_CP036090:238632-238982 [Bacillus toyonensis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGATTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGATTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT119198 WP_000004570.1 NZ_CP036090:238340-238627 [Bacillus toyonensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT119198 NZ_CP036090:238340-238627 [Bacillus toyonensis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACGGTAGAACGCTTAGTAAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References