Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 1155413..1156055 Replicon chromosome
Accession NZ_CP036085
Organism Bacillus mycoides strain JAS12/5

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag EXW46_RS05800 Protein ID WP_000635965.1
Coordinates 1155413..1155763 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag EXW46_RS05805 Protein ID WP_000004570.1
Coordinates 1155768..1156055 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXW46_RS05785 (EXW46_05860) 1152336..1152791 - 456 WP_215576745.1 SprT family protein -
EXW46_RS05790 (EXW46_05865) 1153002..1153118 + 117 WP_001143642.1 cortex morphogenetic protein CmpA -
EXW46_RS05795 (EXW46_05870) 1153176..1155344 - 2169 WP_131248052.1 Tex family protein -
EXW46_RS05800 (EXW46_05875) 1155413..1155763 - 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EXW46_RS05805 (EXW46_05880) 1155768..1156055 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
EXW46_RS05810 (EXW46_05885) 1156367..1157536 - 1170 WP_088034765.1 alanine racemase -
EXW46_RS05815 (EXW46_05890) 1157654..1158604 - 951 WP_002091373.1 outer membrane lipoprotein carrier protein LolA -
EXW46_RS05820 (EXW46_05895) 1158761..1159120 - 360 WP_088034763.1 holo-ACP synthase -
EXW46_RS05825 (EXW46_05900) 1159213..1159785 + 573 WP_113735921.1 rhomboid family intramembrane serine protease -
EXW46_RS05830 (EXW46_05905) 1159778..1160740 - 963 WP_131248046.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T119196 WP_000635965.1 NZ_CP036085:c1155763-1155413 [Bacillus mycoides]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T119196 NZ_CP036085:c1155763-1155413 [Bacillus mycoides]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCGACTGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGGGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT119196 WP_000004570.1 NZ_CP036085:c1156055-1155768 [Bacillus mycoides]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT119196 NZ_CP036085:c1156055-1155768 [Bacillus mycoides]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References