119194

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 240559..241201 Replicon chromosome
Accession NZ_CP036084
Organism Bacillus mycoides strain JAS1004

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag EXW59_RS05895 Protein ID WP_000635965.1
Coordinates 240851..241201 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag EXW59_RS05890 Protein ID WP_000004570.1
Coordinates 240559..240846 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXW59_RS05865 (EXW59_06085) 235874..236836 + 963 WP_088038197.1 UV DNA damage repair endonuclease UvsE -
EXW59_RS05870 (EXW59_06090) 236829..237401 - 573 WP_002167128.1 rhomboid family intramembrane serine protease -
EXW59_RS05875 (EXW59_06095) 237494..237853 + 360 WP_002029438.1 holo-ACP synthase -
EXW59_RS05880 (EXW59_06100) 238010..238960 + 951 WP_002091373.1 outer membrane lipoprotein carrier protein LolA -
EXW59_RS05885 (EXW59_06105) 239078..240247 + 1170 WP_002009971.1 alanine racemase -
EXW59_RS05890 (EXW59_06110) 240559..240846 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
EXW59_RS05895 (EXW59_06115) 240851..241201 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EXW59_RS05900 (EXW59_06120) 241270..243438 + 2169 WP_002029442.1 Tex family protein -
EXW59_RS05905 (EXW59_06125) 243497..243613 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
EXW59_RS05910 (EXW59_06130) 243824..244279 + 456 WP_016095131.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T119194 WP_000635965.1 NZ_CP036084:240851-241201 [Bacillus mycoides]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T119194 NZ_CP036084:240851-241201 [Bacillus mycoides]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCGACTGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGGGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT119194 WP_000004570.1 NZ_CP036084:240559-240846 [Bacillus mycoides]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT119194 NZ_CP036084:240559-240846 [Bacillus mycoides]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References