Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 243896..244538 Replicon chromosome
Accession NZ_CP036073
Organism Bacillus wiedmannii strain JAS08/1

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag EXW45_RS01380 Protein ID WP_000635963.1
Coordinates 244188..244538 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag EXW45_RS01375 Protein ID WP_000004570.1
Coordinates 243896..244183 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXW45_RS01350 (EXW45_01355) 239212..240174 + 963 WP_046956659.1 UV DNA damage repair endonuclease UvsE -
EXW45_RS01355 (EXW45_01360) 240167..240739 - 573 WP_000906923.1 rhomboid family intramembrane serine protease -
EXW45_RS01360 (EXW45_01365) 240832..241191 + 360 WP_097918248.1 holo-ACP synthase -
EXW45_RS01365 (EXW45_01370) 241348..242298 + 951 WP_002096914.1 outer membrane lipoprotein carrier protein LolA -
EXW45_RS01370 (EXW45_01375) 242417..243586 + 1170 WP_048562622.1 alanine racemase -
EXW45_RS01375 (EXW45_01380) 243896..244183 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
EXW45_RS01380 (EXW45_01385) 244188..244538 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EXW45_RS01385 (EXW45_01390) 244607..246775 + 2169 WP_141474917.1 Tex family protein -
EXW45_RS01390 (EXW45_01395) 246834..246950 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
EXW45_RS01395 (EXW45_01400) 247146..247604 + 459 WP_097918246.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T119192 WP_000635963.1 NZ_CP036073:244188-244538 [Bacillus wiedmannii]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T119192 NZ_CP036073:244188-244538 [Bacillus wiedmannii]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CTAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT119192 WP_000004570.1 NZ_CP036073:243896-244183 [Bacillus wiedmannii]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT119192 NZ_CP036073:243896-244183 [Bacillus wiedmannii]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCGGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References