119190

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 245619..246261 Replicon chromosome
Accession NZ_CP036070
Organism Bacillus wiedmannii strain JAS07/5

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag EXW41_RS01390 Protein ID WP_000635963.1
Coordinates 245911..246261 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag EXW41_RS01385 Protein ID WP_000004570.1
Coordinates 245619..245906 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXW41_RS01360 (EXW41_01360) 240935..241897 + 963 WP_088061741.1 UV DNA damage repair endonuclease UvsE -
EXW41_RS01365 (EXW41_01365) 241890..242462 - 573 WP_088061743.1 rhomboid family intramembrane serine protease -
EXW41_RS01370 (EXW41_01370) 242555..242914 + 360 WP_000635044.1 holo-ACP synthase -
EXW41_RS01375 (EXW41_01375) 243071..244021 + 951 WP_002096914.1 outer membrane lipoprotein carrier protein LolA -
EXW41_RS01380 (EXW41_01380) 244140..245309 + 1170 WP_088061745.1 alanine racemase -
EXW41_RS01385 (EXW41_01385) 245619..245906 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
EXW41_RS01390 (EXW41_01390) 245911..246261 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EXW41_RS01395 (EXW41_01395) 246330..248498 + 2169 WP_141542686.1 Tex family protein -
EXW41_RS01400 (EXW41_01400) 248556..248672 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
EXW41_RS01405 (EXW41_01405) 248868..249326 + 459 WP_000344252.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T119190 WP_000635963.1 NZ_CP036070:245911-246261 [Bacillus wiedmannii]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T119190 NZ_CP036070:245911-246261 [Bacillus wiedmannii]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CTAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT119190 WP_000004570.1 NZ_CP036070:245619-245906 [Bacillus wiedmannii]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT119190 NZ_CP036070:245619-245906 [Bacillus wiedmannii]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCGGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References