Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /SpoIISA(toxin)
Location 2466137..2467115 Replicon chromosome
Accession NZ_CP036064
Organism Bacillus mycoides strain JAS06/3

Toxin (Protein)


Gene name spoIISA Uniprot ID R8N1U0
Locus tag EXW39_RS12460 Protein ID WP_002032139.1
Coordinates 2466137..2466877 (+) Length 247 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID R8HWP4
Locus tag EXW39_RS12465 Protein ID WP_000588712.1
Coordinates 2466990..2467115 (+) Length 42 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXW39_RS12430 (EXW39_12670) 2461238..2461999 + 762 WP_033709109.1 ABC transporter ATP-binding protein -
EXW39_RS12435 (EXW39_12675) 2462105..2462815 + 711 Protein_2389 class I SAM-dependent methyltransferase -
EXW39_RS12440 (EXW39_12680) 2462934..2463347 + 414 WP_002032132.1 VOC family protein -
EXW39_RS12445 (EXW39_12685) 2463524..2464156 - 633 WP_002032134.1 type 1 glutamine amidotransferase family protein -
EXW39_RS12450 (EXW39_12690) 2464430..2464906 + 477 WP_002017049.1 MarR family winged helix-turn-helix transcriptional regulator -
EXW39_RS12455 (EXW39_12695) 2465060..2465797 + 738 WP_002032137.1 2,3-diphosphoglycerate-dependent phosphoglycerate mutase -
EXW39_RS12460 (EXW39_12700) 2466137..2466877 + 741 WP_002032139.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
EXW39_RS12465 (EXW39_12705) 2466990..2467115 + 126 WP_000588712.1 hypothetical protein Antitoxin
EXW39_RS12470 2467191..2467367 + 177 WP_002032141.1 stage II sporulation protein SB -
EXW39_RS12475 (EXW39_12710) 2467387..2467755 - 369 WP_033709111.1 YxeA family protein -
EXW39_RS12480 (EXW39_12715) 2468027..2469496 + 1470 WP_002032146.1 beta-Ala-His dipeptidase -
EXW39_RS12485 (EXW39_12720) 2470146..2471340 - 1195 Protein_2399 collagen-like protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(10-244)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 247 a.a.        Molecular weight: 28482.11 Da        Isoelectric Point: 8.3034

>T119189 WP_002032139.1 NZ_CP036064:2466137-2466877 [Bacillus mycoides]
MTISNIRIGLFILAIVFIVLVFFYWRNEELYEEKKQRIRKTWYGLFITSVTVYFTIKGIDLTLWKNLLMFTAMVIFVDIA
FILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPTAFGTMEWHTEEEYTKSLNTFLDSYGEKIGAK
IVVFEAVKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIH
HSYFSK

Download         Length: 741 bp

>T119189 NZ_CP036064:2466137-2466877 [Bacillus mycoides]
TTGACAATCTCTAACATTCGAATTGGCTTATTTATTTTAGCAATCGTCTTTATTGTTCTTGTTTTCTTTTATTGGAGAAA
TGAAGAGTTGTACGAGGAGAAGAAACAACGAATTCGAAAAACGTGGTACGGTTTGTTCATAACATCAGTCACAGTTTATT
TCACGATAAAAGGAATAGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCGATGGTAATTTTCGTTGATATTGCA
TTCATTTTGACACCAAATATTTCAGAAATATGGGGCGCAAAATTCAGCGATATTGGAAAGACGGTCCAATCAATTAAAAG
GTCATTAATTGCTTCAAAAGCAAGAGGAGAAATATATACGACAATTATTCAAAATGTGAATCCAACAGCCTTTGGGACAA
TGGAATGGCATACGGAAGAGGAATATACAAAAAGCTTAAACACATTTTTAGATTCATATGGGGAAAAGATTGGTGCGAAA
ATTGTTGTATTTGAAGCGGTGAAGGAATTAAATACAAACTTTCGTGGTATCCGCTCTCAATTTAGTATTATTGTTCCATT
AGAACACATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTTAGTG
ATGTTTTCATTGTTATTGATGGGAAGAAAAATAACCTTCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATTCAT
CATAGTTATTTTAGTAAATAA

Antitoxin


Download         Length: 42 a.a.        Molecular weight: 4938.34 Da        Isoelectric Point: 3.9150

>AT119189 WP_000588712.1 NZ_CP036064:2466990-2467115 [Bacillus mycoides]
VIKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRK

Download         Length: 126 bp

>AT119189 NZ_CP036064:2466990-2467115 [Bacillus mycoides]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACAGATTTCTCTTTAATAAAAGGAGATAC
TTTGCAGCAAGTAGAAGAGTTAGAAGAAGAATTAAAAAGAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB R8N1U0

Antitoxin

Source ID Structure
AlphaFold DB A0A366GQT1

References