Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 240462..241104 Replicon chromosome
Accession NZ_CP036064
Organism Bacillus mycoides strain JAS06/3

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag EXW39_RS01345 Protein ID WP_000635965.1
Coordinates 240754..241104 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag EXW39_RS01340 Protein ID WP_000004570.1
Coordinates 240462..240749 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXW39_RS01315 (EXW39_01330) 235777..236739 + 963 WP_215590579.1 UV DNA damage repair endonuclease UvsE -
EXW39_RS01320 (EXW39_01335) 236732..237304 - 573 WP_002167128.1 rhomboid family intramembrane serine protease -
EXW39_RS01325 (EXW39_01340) 237397..237756 + 360 WP_088034763.1 holo-ACP synthase -
EXW39_RS01330 (EXW39_01345) 237913..238863 + 951 WP_002124820.1 outer membrane lipoprotein carrier protein LolA -
EXW39_RS01335 (EXW39_01350) 238981..240150 + 1170 WP_215590580.1 alanine racemase -
EXW39_RS01340 (EXW39_01355) 240462..240749 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
EXW39_RS01345 (EXW39_01360) 240754..241104 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EXW39_RS01350 (EXW39_01365) 241173..243341 + 2169 WP_002029442.1 Tex family protein -
EXW39_RS01355 (EXW39_01370) 243400..243516 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
EXW39_RS01360 (EXW39_01375) 243727..244182 + 456 WP_016095131.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T119188 WP_000635965.1 NZ_CP036064:240754-241104 [Bacillus mycoides]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T119188 NZ_CP036064:240754-241104 [Bacillus mycoides]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCGACTGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGGGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT119188 WP_000004570.1 NZ_CP036064:240462-240749 [Bacillus mycoides]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT119188 NZ_CP036064:240462-240749 [Bacillus mycoides]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References