Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 238129..238771 Replicon chromosome
Accession NZ_CP036052
Organism Bacillus toyonensis strain JAS03/3

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag EXW64_RS01355 Protein ID WP_000635963.1
Coordinates 238421..238771 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag EXW64_RS01350 Protein ID WP_000004570.1
Coordinates 238129..238416 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXW64_RS01325 (EXW64_01340) 233614..234576 + 963 WP_098628268.1 UV DNA damage repair endonuclease UvsE -
EXW64_RS01330 (EXW64_01345) 234569..235141 - 573 WP_000908503.1 rhomboid family intramembrane serine protease -
EXW64_RS01335 (EXW64_01350) 235234..235593 + 360 WP_000635048.1 holo-ACP synthase -
EXW64_RS01340 (EXW64_01355) 235750..236700 + 951 WP_215589676.1 outer membrane lipoprotein carrier protein LolA -
EXW64_RS01345 (EXW64_01360) 236818..237987 + 1170 WP_000390621.1 alanine racemase -
EXW64_RS01350 (EXW64_01365) 238129..238416 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
EXW64_RS01355 (EXW64_01370) 238421..238771 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EXW64_RS01360 (EXW64_01375) 238840..241008 + 2169 WP_000426215.1 Tex family protein -
EXW64_RS01365 (EXW64_01380) 241067..241183 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
EXW64_RS01370 (EXW64_01385) 241391..241858 + 468 WP_000544525.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T119180 WP_000635963.1 NZ_CP036052:238421-238771 [Bacillus toyonensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T119180 NZ_CP036052:238421-238771 [Bacillus toyonensis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGATTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGATTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT119180 WP_000004570.1 NZ_CP036052:238129-238416 [Bacillus toyonensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT119180 NZ_CP036052:238129-238416 [Bacillus toyonensis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACGGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References