Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 244344..244986 Replicon chromosome
Accession NZ_CP036046
Organism Bacillus mycoides strain BPN601

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag EXW28_RS01380 Protein ID WP_000635965.1
Coordinates 244636..244986 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag EXW28_RS01375 Protein ID WP_000004570.1
Coordinates 244344..244631 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXW28_RS01350 (EXW28_01360) 239659..240621 + 963 WP_088038197.1 UV DNA damage repair endonuclease UvsE -
EXW28_RS01355 (EXW28_01365) 240614..241186 - 573 WP_002167128.1 rhomboid family intramembrane serine protease -
EXW28_RS01360 (EXW28_01370) 241279..241638 + 360 WP_002029438.1 holo-ACP synthase -
EXW28_RS01365 (EXW28_01375) 241795..242745 + 951 WP_002091373.1 outer membrane lipoprotein carrier protein LolA -
EXW28_RS01370 (EXW28_01380) 242863..244032 + 1170 WP_061675821.1 alanine racemase -
EXW28_RS01375 (EXW28_01385) 244344..244631 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
EXW28_RS01380 (EXW28_01390) 244636..244986 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EXW28_RS01385 (EXW28_01395) 245055..247223 + 2169 WP_215553124.1 Tex family protein -
EXW28_RS01390 (EXW28_01400) 247281..247397 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
EXW28_RS01395 (EXW28_01405) 247608..248063 + 456 WP_215584398.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T119178 WP_000635965.1 NZ_CP036046:244636-244986 [Bacillus mycoides]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T119178 NZ_CP036046:244636-244986 [Bacillus mycoides]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCGACTGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGGGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT119178 WP_000004570.1 NZ_CP036046:244344-244631 [Bacillus mycoides]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT119178 NZ_CP036046:244344-244631 [Bacillus mycoides]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTACCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References