Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /SpoIISA(toxin)
Location 2339997..2340974 Replicon chromosome
Accession NZ_CP036043
Organism Bacillus mycoides strain BPN58/4

Toxin (Protein)


Gene name spoIISA Uniprot ID R8D9S4
Locus tag EXW51_RS12225 Protein ID WP_002069311.1
Coordinates 2339997..2340737 (+) Length 247 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID J8YSJ3
Locus tag EXW51_RS12230 Protein ID WP_002069312.1
Coordinates 2340849..2340974 (+) Length 42 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXW51_RS12200 (EXW51_12455) 2335697..2336458 + 762 WP_215574993.1 ABC transporter ATP-binding protein -
EXW51_RS12205 (EXW51_12460) 2336565..2337275 + 711 WP_215574994.1 class I SAM-dependent methyltransferase -
EXW51_RS12210 (EXW51_12465) 2337394..2337807 + 414 WP_002012925.1 VOC family protein -
EXW51_RS12215 (EXW51_12470) 2338292..2338768 + 477 WP_215574995.1 MarR family winged helix-turn-helix transcriptional regulator -
EXW51_RS12220 (EXW51_12475) 2338922..2339659 + 738 WP_002032137.1 2,3-diphosphoglycerate-dependent phosphoglycerate mutase -
EXW51_RS12225 (EXW51_12480) 2339997..2340737 + 741 WP_002069311.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
EXW51_RS12230 (EXW51_12485) 2340849..2340974 + 126 WP_002069312.1 hypothetical protein Antitoxin
EXW51_RS12235 2341050..2341226 + 177 WP_002017052.1 stage II sporulation protein SB -
EXW51_RS12240 (EXW51_12490) 2341246..2341513 - 268 Protein_2267 YxeA family protein -
EXW51_RS12245 (EXW51_12495) 2341780..2343249 + 1470 WP_215574996.1 aminoacyl-histidine dipeptidase -
EXW51_RS12250 2343609..2343758 + 150 WP_002069316.1 hypothetical protein -
EXW51_RS12255 (EXW51_12500) 2344513..2345172 + 660 WP_215574997.1 CatB-related O-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(10-244)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 247 a.a.        Molecular weight: 28484.21 Da        Isoelectric Point: 8.3034

>T119177 WP_002069311.1 NZ_CP036043:2339997-2340737 [Bacillus mycoides]
MTISNIRIGLFILVIAFVVLVFFYWRNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIA
FILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEMYTTIIQNVNPAAFGTMEWHTEEEYTKSLNSFLDSYGEKIGAK
IVVFEAVKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIH
HSYFSK

Download         Length: 741 bp

>T119177 NZ_CP036043:2339997-2340737 [Bacillus mycoides]
TTGACGATCTCTAACATTCGAATCGGTTTATTTATTCTAGTAATCGCCTTTGTAGTTCTTGTTTTCTTTTATTGGAGAAA
TGAAGAGTTGTACGAGGAGAAGAAACAGCGAATTCGAAAAACGTGGTATGGTTTGTTTATAATATCAGTCACAGTTTATT
TCATGATAAAAGGAATAGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCGATGGTAATTTTCGTTGATATTGCA
TTCATTTTGACACCTAATATTTCAGAAATATGGGGCGCGAAATTCAGCGATATTGGAAAGACGGTCCAATCAATTAAAAG
GTCATTAATTGCTTCAAAAGCAAGAGGAGAAATGTATACGACAATTATTCAAAATGTGAATCCAGCAGCCTTTGGGACAA
TGGAATGGCATACGGAAGAGGAATATACAAAAAGCTTAAACTCATTTTTAGATTCATATGGGGAAAAGATTGGTGCGAAA
ATTGTTGTGTTTGAAGCGGTGAAGGAATTAAATACAAACTTTCGTGGTATCCGCTCTCAATTTAGTATTATTGTTCCATT
AGAACACATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGAATTATACCAGCGAAAATAGTTAGTG
ATGTTTTCATTGTTATTGATGGGAAGAAAAATAATCTTCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATTCAT
CATAGTTATTTTAGTAAATAA

Antitoxin


Download         Length: 42 a.a.        Molecular weight: 4937.36 Da        Isoelectric Point: 3.9798

>AT119177 WP_002069312.1 NZ_CP036043:2340849-2340974 [Bacillus mycoides]
VIKDYDNDFFKENNEDTTDFSLIKGDTLQQVEELEEELKRK

Download         Length: 126 bp

>AT119177 NZ_CP036043:2340849-2340974 [Bacillus mycoides]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAAATAATGAAGATACAACAGATTTCTCTTTAATAAAAGGAGATAC
TTTGCAGCAAGTAGAAGAGTTAGAAGAAGAATTAAAAAGAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB R8D9S4

Antitoxin

Source ID Structure
AlphaFold DB J8YSJ3

References