Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 245916..246558 Replicon chromosome
Accession NZ_CP036043
Organism Bacillus mycoides strain BPN58/4

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag EXW51_RS01760 Protein ID WP_000635965.1
Coordinates 246208..246558 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag EXW51_RS01755 Protein ID WP_000004570.1
Coordinates 245916..246203 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXW51_RS01730 (EXW51_01745) 241231..242193 + 963 WP_002009966.1 UV DNA damage repair endonuclease UvsE -
EXW51_RS01735 (EXW51_01750) 242186..242758 - 573 WP_215574293.1 rhomboid family intramembrane serine protease -
EXW51_RS01740 (EXW51_01755) 242851..243210 + 360 WP_002009969.1 holo-ACP synthase -
EXW51_RS01745 (EXW51_01760) 243367..244317 + 951 WP_002091373.1 outer membrane lipoprotein carrier protein LolA -
EXW51_RS01750 (EXW51_01765) 244435..245604 + 1170 WP_215574294.1 alanine racemase -
EXW51_RS01755 (EXW51_01770) 245916..246203 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
EXW51_RS01760 (EXW51_01775) 246208..246558 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EXW51_RS01765 (EXW51_01780) 246627..248795 + 2169 WP_215575135.1 Tex family protein -
EXW51_RS01770 (EXW51_01785) 248854..248970 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
EXW51_RS01775 (EXW51_01790) 249180..249635 + 456 WP_016095131.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T119176 WP_000635965.1 NZ_CP036043:246208-246558 [Bacillus mycoides]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T119176 NZ_CP036043:246208-246558 [Bacillus mycoides]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCGACTGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGGGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT119176 WP_000004570.1 NZ_CP036043:245916-246203 [Bacillus mycoides]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT119176 NZ_CP036043:245916-246203 [Bacillus mycoides]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References