Detailed information of TA system TA119170

Bioinformatically predicted

Overview

TA module

• Type: III
• Classification (family/domain): toxIN/ToxN(toxin)
• Location: 8725..9455
• Replicon: plasmid p92
• Accession: NZ_CP036037
• Organism: Bacillus mycoides strain BPN57/2

Toxin (Protein)

• Gene name: toxN
• Uniprot ID: -
• Locus tag: EXW62_RS25875
• Protein ID: WP_215556671.1
• Coordinates: 8883..9455 (+)
• Length: 191 a.a.

Antitoxin (RNA)

Gene name	toxI
Locus tag	-
Coordinates	8725..8821 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXW62_RS25850 (3841)	3841..4736	-	896	Protein_4	hypothetical protein	-
EXW62_RS25855 (4757)	4757..5083	-	327	Protein_5	hypothetical protein	-
EXW62_RS25860 (5224)	5224..6655	-	1432	Protein_6	IS4 family transposase	-
EXW62_RS25865 (6894)	6894..7444	+	551	Protein_7	metal-dependent hydrolase	-
EXW62_RS25870 (7610)	7610..8635	+	1026	WP_215556670.1	DUF3991 and TOPRIM domain-containing protein	-
- (8725)	8725..8821	+	97	NuclAT_0	-	Antitoxin
- (8725)	8725..8821	+	97	NuclAT_0	-	Antitoxin
- (8725)	8725..8821	+	97	NuclAT_0	-	Antitoxin
- (8725)	8725..8821	+	97	NuclAT_0	-	Antitoxin
EXW62_RS25875 (8883)	8883..9455	+	573	WP_215556671.1	type III toxin-antitoxin system ToxN/AbiQ family toxin	Toxin
EXW62_RS25885 (10930)	10930..13074	+	2145	WP_215556673.1	type IA DNA topoisomerase	-
EXW62_RS25890 (13109)	13109..13561	+	453	WP_141534564.1	CPBP family intramembrane metalloprotease	-
EXW62_RS28520 (13793)	13793..13924	+	132	WP_255290658.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..92054	92054
	-	inside	IScluster/Tn	-	-	5224..10244	5020

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ToxN/AbiQ toxin (8-171)

Sequences

Toxin

Protein

• Length: 191 a.a.
• Molecular weight: 22339.60 Da
• Isoelectric Point: 9.3674

>T119170 WP_215556671.1 NZ_CP036037:8883-9455 [Bacillus mycoides]
MANKDNPKFYTISTKYIDYLRETDSKVPFNKDEQHSRPYVGVLEKINGHDYFVPLTSRNDKNLNSQVSVKLFDNDNPEKR
IGVLLVNNMIPVPEKECKEIDIAEKTETDPKYGNLMLKQYLFLKENMDRVSNKVEKVYKDVTVQGKPSHKQKFLKGVCCD
FSKLEEKCQEYKEKGQARRIAYMRQMGRER

Nucleotide

• Length: 573 bp

>T119170 NZ_CP036037:8883-9455 [Bacillus mycoides]
TTGGCTAATAAAGATAATCCTAAATTTTACACTATTTCAACTAAATATATAGACTATCTAAGAGAAACAGATAGTAAAGT
ACCCTTTAATAAAGACGAACAGCATAGCAGACCGTATGTAGGTGTATTGGAAAAAATCAATGGTCATGATTATTTTGTGC
CTTTAACATCACGTAACGATAAAAATCTTAATAGCCAGGTCAGTGTAAAGTTATTCGATAATGATAACCCTGAGAAGCGA
ATTGGAGTTTTATTGGTCAATAACATGATTCCAGTCCCAGAAAAAGAGTGTAAAGAAATAGATATAGCGGAAAAAACGGA
GACAGATCCTAAGTATGGTAATTTGATGTTAAAACAGTATTTGTTTTTAAAAGAGAATATGGATCGAGTTTCTAATAAAG
TGGAAAAAGTATATAAAGATGTAACTGTACAAGGAAAGCCTTCACATAAACAGAAATTCTTAAAAGGAGTTTGCTGTGAT
TTCTCTAAATTAGAGGAAAAGTGCCAGGAATATAAAGAAAAGGGTCAAGCTAGAAGAATAGCATATATGCGCCAAATGGG
GCGTGAGAGATAA

Antitoxin

Nucleotide

• Length: 97 bp

>AT119170 NZ_CP036037:8725-8821 [Bacillus mycoides]
GTAGCCTTACTTTAGTAGGCGCGAAAAAATTGGTGTAACTCTACCGTAGTGAGTACCAAAAAATTGGTGTAGCCTTACCG
TAGTAGGCACAAAAAAA

Structures

Antitoxin