Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 240308..240950 Replicon chromosome
Accession NZ_CP036034
Organism Bacillus mycoides strain BPN57/2

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag EXW62_RS01345 Protein ID WP_000635965.1
Coordinates 240600..240950 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag EXW62_RS01340 Protein ID WP_000004570.1
Coordinates 240308..240595 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXW62_RS01315 (EXW62_01325) 235466..236428 + 963 WP_215555025.1 UV DNA damage repair endonuclease UvsE -
EXW62_RS01320 (EXW62_01330) 236601..237149 - 549 WP_215555026.1 rhomboid family intramembrane serine protease -
EXW62_RS01325 (EXW62_01335) 237242..237601 + 360 WP_215555027.1 holo-ACP synthase -
EXW62_RS01330 (EXW62_01340) 237758..238708 + 951 WP_215556550.1 outer membrane lipoprotein carrier protein LolA -
EXW62_RS01335 (EXW62_01345) 238826..239995 + 1170 WP_215555028.1 alanine racemase -
EXW62_RS01340 (EXW62_01350) 240308..240595 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
EXW62_RS01345 (EXW62_01355) 240600..240950 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EXW62_RS01350 (EXW62_01360) 241019..243187 + 2169 WP_215556551.1 Tex family protein -
EXW62_RS01355 (EXW62_01365) 243245..243361 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
EXW62_RS01360 (EXW62_01370) 243571..244026 + 456 WP_215555029.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T119168 WP_000635965.1 NZ_CP036034:240600-240950 [Bacillus mycoides]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T119168 NZ_CP036034:240600-240950 [Bacillus mycoides]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCGACTGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTCGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT119168 WP_000004570.1 NZ_CP036034:240308-240595 [Bacillus mycoides]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT119168 NZ_CP036034:240308-240595 [Bacillus mycoides]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References