Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 243649..244291 Replicon chromosome
Accession NZ_CP036026
Organism Bacillus mycoides strain BPN54/2

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag EXW38_RS01375 Protein ID WP_000635965.1
Coordinates 243941..244291 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag EXW38_RS01370 Protein ID WP_000004570.1
Coordinates 243649..243936 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXW38_RS01345 (EXW38_01360) 238895..239857 + 963 WP_002159536.1 UV DNA damage repair endonuclease UvsE -
EXW38_RS01350 (EXW38_01365) 239942..240490 - 549 WP_215572279.1 rhomboid family intramembrane serine protease -
EXW38_RS01355 (EXW38_01370) 240583..240942 + 360 WP_002009969.1 holo-ACP synthase -
EXW38_RS01360 (EXW38_01375) 241099..242049 + 951 WP_078180266.1 outer membrane lipoprotein carrier protein LolA -
EXW38_RS01365 (EXW38_01380) 242167..243336 + 1170 WP_002159533.1 alanine racemase -
EXW38_RS01370 (EXW38_01385) 243649..243936 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
EXW38_RS01375 (EXW38_01390) 243941..244291 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EXW38_RS01380 (EXW38_01395) 244360..246528 + 2169 WP_016103364.1 Tex family protein -
EXW38_RS01385 (EXW38_01400) 246586..246702 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
EXW38_RS01390 (EXW38_01405) 246912..247367 + 456 WP_002159531.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T119165 WP_000635965.1 NZ_CP036026:243941-244291 [Bacillus mycoides]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T119165 NZ_CP036026:243941-244291 [Bacillus mycoides]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCGACTGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT119165 WP_000004570.1 NZ_CP036026:243649-243936 [Bacillus mycoides]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT119165 NZ_CP036026:243649-243936 [Bacillus mycoides]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References