Detailed information of TA system TA119164

Bioinformatically predicted

Overview

TA module

• Type: VII
• Classification (family/domain): HepT-MntA/HepT(toxin)
• Location: 451355..452165
• Replicon: plasmid p479
• Accession: NZ_CP036025
• Organism: Bacillus mycoides strain BPN52/2

Toxin (Protein)

• Gene name: hepT
• Uniprot ID: -
• Locus tag: EXW49_RS29340
• Protein ID: WP_215589355.1
• Coordinates: 451355..451768 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: mntA
• Uniprot ID: C2Y2N7
• Locus tag: EXW49_RS29345
• Protein ID: WP_002068816.1
• Coordinates: 451758..452165 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXW49_RS29320 (EXW49_29945)	447234..447542	-	309	WP_201055060.1	metalloregulator ArsR/SmtB family transcription factor	-
EXW49_RS29325 (EXW49_29950)	448263..448616	+	354	WP_201055054.1	hypothetical protein	-
EXW49_RS29330 (EXW49_29955)	449031..450128	-	1098	WP_215589354.1	S-layer homology domain-containing protein	-
EXW49_RS29335 (EXW49_29960)	450436..450651	-	216	WP_001167049.1	alpha/beta-type small acid-soluble spore protein	-
EXW49_RS29340 (EXW49_29965)	451355..451768	-	414	WP_215589355.1	DUF86 domain-containing protein	Toxin
EXW49_RS29345 (EXW49_29970)	451758..452165	-	408	WP_002068816.1	nucleotidyltransferase domain-containing protein	Antitoxin
EXW49_RS29350 (EXW49_29975)	452777..453757	-	981	WP_002068814.1	hypothetical protein	-
EXW49_RS29355 (EXW49_29980)	453886..454629	-	744	WP_002068812.1	hypothetical protein	-
EXW49_RS29360 (EXW49_29985)	454629..455450	-	822	WP_002068811.1	DUF692 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..479471	479471

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease HepT-like (13-130)

Antitoxin

• Polymerase beta, nucleotidyltransferase (10-99)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15527.08 Da
• Isoelectric Point: 6.2332

>T119164 WP_215589355.1 NZ_CP036025:c451768-451355 [Bacillus mycoides]
MKNEVILNKISTIERCLKRIQDVYGNDPDNLEDYTKQDSIILNIQRACEASIDLAMHIVAGKKLGLPQSSREAFDLLVTA
DLLSAELANKLKAMVGFRNIAVHDYQSVNLDIVRQIIEKHLKDFKLFTKEVMGILDL

Nucleotide

• Length: 414 bp

>T119164 NZ_CP036025:c451768-451355 [Bacillus mycoides]
ATGAAGAATGAAGTTATTTTGAATAAAATTTCTACAATAGAGCGTTGTTTAAAAAGAATTCAAGATGTATATGGAAATGA
CCCTGATAATTTAGAGGATTATACTAAGCAAGACTCTATCATATTGAACATACAAAGAGCTTGCGAAGCAAGTATTGATT
TAGCAATGCATATTGTCGCAGGGAAAAAATTAGGATTACCTCAATCGAGTCGAGAGGCTTTTGATTTGTTGGTTACAGCA
GATTTACTTAGCGCTGAGCTAGCAAATAAGTTAAAGGCAATGGTTGGATTTCGAAATATAGCTGTTCATGATTATCAAAG
TGTAAATTTAGATATTGTTCGTCAAATTATTGAAAAACATCTAAAAGACTTTAAACTGTTTACGAAAGAAGTTATGGGAA
TTCTGGACTTATAG

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 15703.91 Da
• Isoelectric Point: 4.4169

>AT119164 WP_002068816.1 NZ_CP036025:c452165-451758 [Bacillus mycoides]
MIFQSMITAIIEKLQETVNPYWIILFGSTAENRAREDSDIDIAYLSDVTLGNYERFLLAQELANIVHRDVDLVDLSEAST
VFQAQIIHTGKTIFCSDEERKVIFEMKTLKMYSKLNEERQIVFDDIKKRGSIYEE

Nucleotide

• Length: 408 bp

>AT119164 NZ_CP036025:c452165-451758 [Bacillus mycoides]
ATGATTTTTCAAAGCATGATTACAGCTATTATAGAAAAATTACAAGAGACGGTGAATCCCTATTGGATAATTTTGTTTGG
ATCAACAGCGGAGAACAGAGCACGAGAAGATAGTGATATAGATATAGCCTATTTAAGTGACGTAACTTTAGGTAATTATG
AACGTTTTTTATTAGCACAGGAGTTAGCAAACATCGTTCATAGAGATGTTGATTTAGTTGATTTATCAGAAGCTTCGACA
GTTTTTCAAGCTCAGATTATACATACAGGAAAGACAATATTTTGTTCCGATGAAGAACGAAAGGTCATTTTTGAAATGAA
GACTTTAAAAATGTATTCAAAATTGAATGAAGAACGCCAGATAGTATTTGATGATATAAAGAAGAGAGGTTCTATATATG
AAGAATGA

Structures

Antitoxin

• AlphaFold DB: C2Y2N7