Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 242499..243141 Replicon chromosome
Accession NZ_CP036023
Organism Bacillus mycoides strain BPN52/2

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag EXW49_RS01365 Protein ID WP_000635965.1
Coordinates 242791..243141 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag EXW49_RS01360 Protein ID WP_000004570.1
Coordinates 242499..242786 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXW49_RS01335 (EXW49_01350) 237662..238624 + 963 WP_002029436.1 UV DNA damage repair endonuclease UvsE -
EXW49_RS01340 (EXW49_01355) 238794..239342 - 549 WP_215588675.1 rhomboid family intramembrane serine protease -
EXW49_RS01345 (EXW49_01360) 239434..239793 + 360 WP_002009969.1 holo-ACP synthase -
EXW49_RS01350 (EXW49_01365) 239950..240900 + 951 WP_002124820.1 outer membrane lipoprotein carrier protein LolA -
EXW49_RS01355 (EXW49_01370) 241018..242187 + 1170 WP_002009971.1 alanine racemase -
EXW49_RS01360 (EXW49_01375) 242499..242786 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
EXW49_RS01365 (EXW49_01380) 242791..243141 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EXW49_RS01370 (EXW49_01385) 243210..245378 + 2169 WP_002029442.1 Tex family protein -
EXW49_RS01375 (EXW49_01390) 245436..245552 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
EXW49_RS01380 (EXW49_01395) 245763..246218 + 456 WP_016095131.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T119162 WP_000635965.1 NZ_CP036023:242791-243141 [Bacillus mycoides]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T119162 NZ_CP036023:242791-243141 [Bacillus mycoides]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCGACTGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGGGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTAGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT119162 WP_000004570.1 NZ_CP036023:242499-242786 [Bacillus mycoides]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT119162 NZ_CP036023:242499-242786 [Bacillus mycoides]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References