119160

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 246137..246779 Replicon chromosome
Accession NZ_CP036017
Organism Bacillus mycoides strain BPN51/1

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag EXW52_RS01370 Protein ID WP_000635965.1
Coordinates 246429..246779 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag EXW52_RS01365 Protein ID WP_000004570.1
Coordinates 246137..246424 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXW52_RS01340 (EXW52_01355) 241299..242261 + 963 WP_061689866.1 UV DNA damage repair endonuclease UvsE -
EXW52_RS01345 (EXW52_01360) 242432..242980 - 549 WP_002139859.1 rhomboid family intramembrane serine protease -
EXW52_RS01350 (EXW52_01365) 243072..243431 + 360 WP_002009969.1 holo-ACP synthase -
EXW52_RS01355 (EXW52_01370) 243588..244538 + 951 WP_002091373.1 outer membrane lipoprotein carrier protein LolA -
EXW52_RS01360 (EXW52_01375) 244656..245825 + 1170 WP_061689867.1 alanine racemase -
EXW52_RS01365 (EXW52_01380) 246137..246424 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
EXW52_RS01370 (EXW52_01385) 246429..246779 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EXW52_RS01375 (EXW52_01390) 246848..249016 + 2169 WP_002029442.1 Tex family protein -
EXW52_RS01380 (EXW52_01395) 249074..249190 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
EXW52_RS01385 (EXW52_01400) 249401..249856 + 456 WP_016095131.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T119160 WP_000635965.1 NZ_CP036017:246429-246779 [Bacillus mycoides]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T119160 NZ_CP036017:246429-246779 [Bacillus mycoides]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCGACTGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGGGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT119160 WP_000004570.1 NZ_CP036017:246137-246424 [Bacillus mycoides]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT119160 NZ_CP036017:246137-246424 [Bacillus mycoides]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References