Detailed information of TA system TA119159

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 4767500..4768478
• Replicon: chromosome
• Accession: NZ_CP036014
• Organism: Bacillus toyonensis strain BPN45/4

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: K0FNW8
• Locus tag: EXW33_RS24260
• Protein ID: WP_000155917.1
• Coordinates: 4767500..4768240 (+)
• Length: 247 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: J8KE32
• Locus tag: EXW33_RS24265
• Protein ID: WP_000588713.1
• Coordinates: 4768353..4768478 (+)
• Length: 42 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXW33_RS24240 (4763160)	4763160..4763942	+	783	WP_000381781.1	class I SAM-dependent methyltransferase	-
EXW33_RS24245 (4764123)	4764123..4765799	-	1677	WP_000857157.1	alpha-keto acid decarboxylase family protein	-
EXW33_RS24250 (4765926)	4765926..4766408	+	483	WP_000191892.1	MarR family winged helix-turn-helix transcriptional regulator	-
EXW33_RS24255 (4766576)	4766576..4767313	+	738	WP_000594132.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
EXW33_RS24260 (4767500)	4767500..4768240	+	741	WP_000155917.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
EXW33_RS24265 (4768353)	4768353..4768478	+	126	WP_000588713.1	hypothetical protein	Antitoxin
EXW33_RS24270 (4768554)	4768554..4768730	+	177	WP_000808012.1	stage II sporulation protein SB	-
EXW33_RS24275 (4768779)	4768779..4769168	-	390	WP_000732294.1	YxeA family protein	-
EXW33_RS24280 (4769412)	4769412..4770881	+	1470	WP_000287564.1	beta-Ala-His dipeptidase	-
EXW33_RS24285 (4771192)	4771192..4772001	+	810	WP_000678381.1	papain-like cysteine protease family protein	-
EXW33_RS24290 (4772029)	4772029..4772631	+	603	WP_000517300.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-244)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 247 a.a.
• Molecular weight: 28446.03 Da
• Isoelectric Point: 7.3946

>T119159 WP_000155917.1 NZ_CP036014:4767500-4768240 [Bacillus toyonensis]
MTISNIRIGLFILAIVFVVLVFFYWRNEELYEEKKQRIRKTWYGLFITSVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIS
FILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTNIIQNINPAAFGTMEWHTEEEYTKSLNTFLDSYGEKIGAK
IVVFEAANELNTGFRGIRSQFSITIPIEHIEQLNEQKAVQVENVGIIPAKIMNDVFIVIDGKKNSLQDRDFENVYNLTIH
HSYFSK

Nucleotide

• Length: 741 bp

>T119159 NZ_CP036014:4767500-4768240 [Bacillus toyonensis]
TTGACGATCTCTAACATTCGAATTGGCTTATTTATTTTAGCAATCGTTTTTGTAGTTCTTGTTTTCTTTTATTGGAGAAA
TGAAGAGTTGTATGAAGAGAAAAAGCAGCGAATTCGAAAAACTTGGTATGGATTATTCATAACATCAGTCACAGTTTATT
TCATGATTAAAGGAATCGATTTAACCCTCTGGAAAAATTTATTAATGTTCACTGCGATGGTCATTTTCGTTGATATTTCA
TTTATTTTAACACCTAACATTTCGGAAATATGGGGAGCGAAATTTAGCGATATCGGTAAAACTGTTCAATCCATAAAGCG
GTCATTAATCGCTTCAAAAGCAAGAGGGGAAATATATACGAACATTATCCAAAATATTAATCCAGCAGCATTTGGGACGA
TGGAATGGCATACGGAAGAGGAATATACAAAAAGCTTAAACACATTTTTAGATTCATATGGGGAAAAAATCGGAGCGAAA
ATTGTTGTATTTGAAGCAGCGAATGAATTAAATACGGGCTTTCGTGGTATTCGTTCTCAATTTAGTATTACAATACCTAT
AGAGCATATAGAGCAATTGAATGAACAAAAAGCAGTACAAGTAGAAAACGTGGGAATTATACCAGCAAAAATAATGAATG
ATGTTTTTATCGTTATTGATGGGAAGAAGAATAGCCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACAT
CATAGTTATTTTAGTAAGTAG

Antitoxin

Protein

• Length: 42 a.a.
• Molecular weight: 4951.43 Da
• Isoelectric Point: 4.1079

>AT119159 WP_000588713.1 NZ_CP036014:4768353-4768478 [Bacillus toyonensis]
VIKDYDNDFFKEENEDTTDFSLIKGDTLQQVEKLEEELKRK

Nucleotide

• Length: 126 bp

>AT119159 NZ_CP036014:4768353-4768478 [Bacillus toyonensis]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGAAAATGAAGATACAACAGATTTCTCTTTAATCAAAGGAGATAC
GTTGCAGCAAGTAGAAAAGTTAGAAGAAGAATTAAAAAGAAAATGA

Structures

Toxin

• AlphaFold DB: K0FNW8

Antitoxin

• AlphaFold DB: J8KE32