Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2664003..2664645 Replicon chromosome
Accession NZ_CP036014
Organism Bacillus toyonensis strain BPN45/4

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag EXW33_RS13810 Protein ID WP_000635963.1
Coordinates 2664295..2664645 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag EXW33_RS13805 Protein ID WP_000004570.1
Coordinates 2664003..2664290 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXW33_RS13780 (2659330) 2659330..2660292 + 963 WP_000961174.1 UV DNA damage repair endonuclease UvsE -
EXW33_RS13785 (2660285) 2660285..2660857 - 573 WP_000908503.1 rhomboid family intramembrane serine protease -
EXW33_RS13790 (2660950) 2660950..2661309 + 360 WP_000635048.1 holo-ACP synthase -
EXW33_RS13795 (2661466) 2661466..2662416 + 951 WP_002037834.1 outer membrane lipoprotein carrier protein LolA -
EXW33_RS13800 (2662534) 2662534..2663703 + 1170 WP_000390621.1 alanine racemase -
EXW33_RS13805 (2664003) 2664003..2664290 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
EXW33_RS13810 (2664295) 2664295..2664645 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EXW33_RS13815 (2664714) 2664714..2666882 + 2169 WP_000426215.1 Tex family protein -
EXW33_RS13820 (2666941) 2666941..2667057 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
EXW33_RS13825 (2667265) 2667265..2667732 + 468 WP_000544525.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T119158 WP_000635963.1 NZ_CP036014:2664295-2664645 [Bacillus toyonensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T119158 NZ_CP036014:2664295-2664645 [Bacillus toyonensis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGATTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGATTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT119158 WP_000004570.1 NZ_CP036014:2664003-2664290 [Bacillus toyonensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT119158 NZ_CP036014:2664003-2664290 [Bacillus toyonensis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACGGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References