Detailed information of TA system TA119154

Bioinformatically predicted

Overview

TA module

• Type: III
• Classification (family/domain): toxIN/ToxN(toxin)
• Location: 46451..47193
• Replicon: chromosome
• Accession: NZ_CP036014
• Organism: Bacillus toyonensis strain BPN45/4

Toxin (Protein)

• Gene name: toxN
• Uniprot ID: -
• Locus tag: EXW33_RS00255
• Protein ID: WP_215599952.1
• Coordinates: 46609..47193 (+)
• Length: 195 a.a.

Antitoxin (RNA)

Gene name	toxI
Locus tag	-
Coordinates	46451..46547 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXW33_RS00225 (41799)	41799..42227	+	429	WP_215599944.1	DUF5514 family protein	-
EXW33_RS00230 (42250)	42250..42459	+	210	WP_000081343.1	DUF5511 family protein	-
EXW33_RS00235 (42456)	42456..43628	+	1173	WP_215599946.1	ArdC family protein	-
EXW33_RS00240 (43692)	43692..44678	+	987	WP_215599947.1	hypothetical protein	-
EXW33_RS00245 (44702)	44702..45253	+	552	WP_215599948.1	metal-dependent hydrolase	-
EXW33_RS00250 (45333)	45333..46358	+	1026	WP_215599950.1	DUF3991 and TOPRIM domain-containing protein	-
- (46451)	46451..46547	+	97	NuclAT_0	-	Antitoxin
- (46451)	46451..46547	+	97	NuclAT_0	-	Antitoxin
- (46451)	46451..46547	+	97	NuclAT_0	-	Antitoxin
- (46451)	46451..46547	+	97	NuclAT_0	-	Antitoxin
EXW33_RS00255 (46609)	46609..47193	+	585	WP_215599952.1	type III toxin-antitoxin system ToxN/AbiQ family toxin	Toxin
EXW33_RS00260 (47517)	47517..49658	+	2142	WP_215599953.1	type IA DNA topoisomerase	-
EXW33_RS00265 (49688)	49688..50134	+	447	WP_215599955.1	CPBP family intramembrane metalloprotease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	17005..107240	90235

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ToxN/AbiQ toxin (8-169)

Sequences

Toxin

Protein

• Length: 195 a.a.
• Molecular weight: 22850.08 Da
• Isoelectric Point: 8.9694

>T119154 WP_215599952.1 NZ_CP036014:46609-47193 [Bacillus toyonensis]
MANKDNPKFYTISTEYIDYLRETDSKVPFNKDEQHSRPYVGVLEKINGHDYFVPLTSRNDKNFNSQVSVKLFDNDEKRIG
VLLVNNMIPVPEKECKEIDIAEKTAADPQYGNLMLKQYLFLKENMDRVSNKVEKVYKDVTVEGKPSHKQKFLKGVCCDFT
KLEEKCQEYKGRDQAKERDKARRMAYMRQMGRER

Nucleotide

• Length: 585 bp

>T119154 NZ_CP036014:46609-47193 [Bacillus toyonensis]
TTGGCTAATAAAGATAATCCTAAATTCTACACCATTTCAACTGAATATATAGATTATTTAAGGGAAACGGATAGTAAAGT
ACCTTTTAATAAAGATGAACAGCATAGTAGACCGTATGTAGGTGTATTGGAAAAAATCAATGGTCATGATTATTTTGTAC
CTTTGACATCACGTAACGATAAAAATTTTAATAGTCAGGTCAGTGTAAAATTATTCGATAATGATGAGAAGCGAATTGGG
GTTTTATTGGTCAATAACATGATTCCAGTCCCAGAAAAAGAGTGTAAAGAAATTGATATAGCAGAAAAAACGGCAGCAGA
CCCTCAGTATGGTAATTTGATGTTAAAACAGTATTTATTTTTAAAAGAAAATATGGATCGAGTTTCTAATAAAGTGGAAA
AAGTATATAAAGATGTAACTGTAGAAGGAAAGCCTTCACATAAACAAAAATTCTTAAAAGGAGTTTGTTGTGATTTCACT
AAATTAGAAGAAAAATGCCAAGAATATAAAGGCAGAGATCAAGCTAAAGAAAGAGATAAAGCTAGAAGAATGGCATATAT
GCGTCAAATGGGGCGCGAGAGATAA

Antitoxin

Nucleotide

• Length: 97 bp

>AT119154 NZ_CP036014:46451-46547 [Bacillus toyonensis]
GTAACCTTACTTTAGTAGGTGCGAAAAAATTGGTGTAACTCTACCGTAGTGAGTACCAAAAAATTGGTGTAGCCTTACCG
TAGTAGGCGCTAAAAAA

Structures

Antitoxin