Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 243644..244286 Replicon chromosome
Accession NZ_CP036009
Organism Bacillus mycoides strain BPN43/2

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag EXW40_RS01365 Protein ID WP_000635965.1
Coordinates 243936..244286 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag EXW40_RS01360 Protein ID WP_000004570.1
Coordinates 243644..243931 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXW40_RS01335 (EXW40_01350) 238960..239922 + 963 WP_002009966.1 UV DNA damage repair endonuclease UvsE -
EXW40_RS01340 (EXW40_01355) 239915..240487 - 573 WP_002167128.1 rhomboid family intramembrane serine protease -
EXW40_RS01345 (EXW40_01360) 240579..240938 + 360 WP_002009969.1 holo-ACP synthase -
EXW40_RS01350 (EXW40_01365) 241095..242045 + 951 WP_002091373.1 outer membrane lipoprotein carrier protein LolA -
EXW40_RS01355 (EXW40_01370) 242163..243332 + 1170 WP_002009971.1 alanine racemase -
EXW40_RS01360 (EXW40_01375) 243644..243931 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
EXW40_RS01365 (EXW40_01380) 243936..244286 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EXW40_RS01370 (EXW40_01385) 244355..246523 + 2169 WP_002029442.1 Tex family protein -
EXW40_RS01375 (EXW40_01390) 246581..246697 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
EXW40_RS01380 (EXW40_01395) 246908..247363 + 456 WP_215587167.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T119152 WP_000635965.1 NZ_CP036009:243936-244286 [Bacillus mycoides]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T119152 NZ_CP036009:243936-244286 [Bacillus mycoides]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCGACTGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGGGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT119152 WP_000004570.1 NZ_CP036009:243644-243931 [Bacillus mycoides]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT119152 NZ_CP036009:243644-243931 [Bacillus mycoides]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References